FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800312
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG24660.30812982936654704No Hit
GATTATTACTGTGCATCATGGGATGACAGCCTGAATGGTCGGGTGTTCGG17260.21566589030278194No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17220.21516608522676156No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16640.20791891162446643No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15700.19617349233798817No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13430.1678095542738332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.16031247813352792No Hit
GTGTATTACTGTGCGCGAGATCGAGGTAGTGGCTACGATTCAGACTACTA11790.14731754615699877No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCATTCAGG10760.1344475654494747No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10050.12557602535011347No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9270.11582982636771659No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9230.11533002129169624No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG9110.11383060606363518No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8570.10708323753736042No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8350.1043343096192485No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC450.00991193647.992196
GAATACG3150.041.136169
GTGCTAG4300.035.262821
CGTATTG850.002838531733.876843
TACACGG5200.029.0721916
GCTAGAG5300.028.523663
TATTGAG7600.028.4164315
GTACACG5450.027.7386055
ATTGAGT5950.026.6175176
GGTTTAT7400.025.3692591
ACGGGGT6300.025.1387659
GTAATAC9800.025.0506521
TGCTAGA6650.024.9215582
GTATGTA9400.024.5803641
GTGTAGA4750.024.3216251
CAGCCGT9500.024.2486869
ACACGGG6550.024.1792747
GTATCAA47500.024.1696131
TCGTATT1500.001548442624.0186042
CACGGGG6950.023.8234628
Produced by FastQC (version 0.11.5)