FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800312
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAGCCTGAATGGTCGGGTGTTCGG16640.20791891162446643No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14520.18142924259538779No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14100.1761812892971741No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA13190.16481072381771109No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG12420.15518947610431932No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11970.14956666899909035No Hit
GTGTATTACTGTGCGCGAGATCGAGGTAGTGGCTACGATTCAGACTACTA10650.13307310149041873No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC10020.1252011715430982No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT9750.1218274872799608No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8330.10408440708123833No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCATTCAGG8020.10021091774208059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA1151.493554E-743.8166926
TTAAGCG800.002108944635.9922833
GAATACG2700.034.6592379
AGTACGG1402.8831442E-530.8505295
GTACACG5500.030.1026385
TACACGG5900.029.2818586
CAGCCGT9450.028.9461779
ACGGGGT5750.028.7938299
GTGCTAG3550.028.4220181
GTAATAC10550.028.0082931
ACACGGG6350.027.2067667
CACGGGG6450.025.6689178
ATGGGAG11500.025.038115
TACACCG2351.4151319E-624.5053865
GTCCTAC7200.024.0233751
TACGGAT1500.001557387923.9948567
GTATTAG3652.3646862E-1023.6942841
ACAGCCG16200.023.5505078
CTAGAGA5250.023.3092884
GCTAGAG4700.022.9737993
Produced by FastQC (version 0.11.5)