FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931809
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATAATCCGTCCCTCAAGAGCCGAGTCACCATATCAGTAGCCACGTCC48090.5160928902811628No Hit
GTAATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGA43510.4669411864448615No Hit
GTCTCTGGTGGCGCCATCAGTACTAGTAATTACTACTGGGGCTGGATCCG41220.44236533452671095No Hit
GTATATTACTGTGCGAGACAGGATAGGAACTGGTTCGATACTTGGGGCCA36400.390637995554883No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29630.31798362110690065No Hit
CCTTAATAATTACTTATTCACTTTCGGCCCTGGGACCACAGTGGCTGTCA25210.27054900736095056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24150.25917328551237434No Hit
GTACTAGTAATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGA23050.24736829114121026No Hit
GCCGTATATTACTGTGCGAGACAGGATAGGAACTGGTTCGATACTTGGGG22910.24586583731215306No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22620.2427536115233916No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC21830.23427547920228287No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21730.23320229789581343No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21210.22762175510217222No Hit
GGATAGGAACTGGTTCGATACTTGGGGCCAGGGAATCCTGGTCACCGTCT20280.21764116895200628No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT19830.2128118530728937No Hit
ATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAACT18910.20293858505337467No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18740.2011141768323766No Hit
TTATTAAGGTGCTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT18150.1947824071242068No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA17790.19091895442091672No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG17700.1899530912450942No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17650.1894165005918595No Hit
CTTTTATAGTGGGAGCACCTACTATAATCCGTCCCTCAAGAGCCGAGTCA17320.1858750022805103No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTG16990.18233350396916106No Hit
CACCTGCACTGTCTCTGGTGGCGCCATCAGTACTAGTAATTACTACTGGG16630.178470051265871No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC16460.17664564304487293No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16200.17385537164805234No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA15870.17031387333670311No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15760.16913337389958671No Hit
ATATTACTGTGCGAGACAGGATAGGAACTGGTTCGATACTTGGGGCCAGG14930.1602259690558902No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14770.15850887896553906No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14640.15711374326712876No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC14530.15593324383001236No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14490.1555039713074246No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14370.15421615373966124No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA14070.15099660982025284No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG13880.14895756533796087No Hit
CCATATCAGTAGCCACGTCCAGGAACCAGTTCTCCCTGAGGCTGACCTCT13860.148742929076667No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13570.14563070328790556No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13440.14423556758949527No Hit
GATTATAGTAGGTGCTCCCACTATAAAAGATACTTCCAATCCACTCCAGT13200.14165993245396857No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC13180.14144529619267468No Hit
GTTCCTATCCTGTCTCGCACAGTAATATACGGCCGTGTCCGCGGCGGTCA13140.1410160236700869No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12940.13886966105714796No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA12500.13414766330868236No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12450.13361107265544764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11910.12781589360051254No Hit
GTCATGGACCTCCTGTGCAAGAACATCAAGCACCTGTGGTTCTTCCTCCT11830.126957348555337No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11710.12566953098757364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11640.12491830407304501No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11420.12255730519881221No Hit
GTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG11350.1218060782842836No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGGCAGAGTCACCATCGCTT11150.11965971567134467No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11140.11955239754069771No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11010.11815726184228742No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10810.1160108992293485No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGC10780.11568894483740767No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10710.11493771792287905No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10670.11450844540029124No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA10610.11386453661640958No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10350.111074265219589No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGACCTCCTGTG10330.1108596289582951No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG10270.11021572017441343No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10230.10978644765182563No Hit
CCCTGAGGCTGACCTCTGTGACCGCCGCGGACACGGCCGTATATTACTGT9930.10656690373241726No Hit
CTATAATCCGTCCCTCAAGAGCCGAGTCACCATATCAGTAGCCACGTCCA9890.10613763120982948No Hit
GTGCAAGAACATCAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC9700.10409858672753751No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9630.10334735981300888No Hit
GTAGGAGGCAGAGTCACCATCGCTTGCCGGGCCAGTCAGGACATTAGCAA9610.10313272355171499No Hit
CAGTAATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGA9520.1021668603758925No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9460.10152295159201082No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCGGA9460.10152295159201082No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9420.10109367906942301No Hit
CCTTGGGGCTGGTCGGGGATGCGGAAGAGACGGTGACCAGGATTCCCTGG9320.10002049776295358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20750.069.081734
ATCAACG21300.067.297933
CAACGCA21700.066.053875
AACGCAG22750.062.95796
ACGCAGA26550.053.9469767
TACTACG553.324235E-452.384435
CGCAGAG27350.052.3690078
TACGGAC450.00989054648.0190625
GTATCAA34850.042.0105441
TATCAAC39450.036.883542
TACATGG27700.036.1462552
GTACATG28100.035.932441
GTATATA3350.034.446111
AGGGGTG8550.030.3278275
GTTCCGC950.00489835130.3050466
CATGGGG17900.029.7787844
GAGTTTA4050.028.4924621
GCAGAGT52000.027.8209089
ATTCAGG9900.027.6830161
GTTAGAC1050.00795539627.4409373
Produced by FastQC (version 0.11.5)