FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931809
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGA40480.4344237928588369No Hit
ACTATAATCCGTCCCTCAAGAGCCGAGTCACCATATCAGTAGCCACGTCC39450.42337002540220153No Hit
GTCTCTGGTGGCGCCATCAGTACTAGTAATTACTACTGGGGCTGGATCCG39250.4212236627892626No Hit
GTATATTACTGTGCGAGACAGGATAGGAACTGGTTCGATACTTGGGGCCA38120.40909671402615777No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28050.3010273564646832No Hit
CCTTAATAATTACTTATTCACTTTCGGCCCTGGGACCACAGTGGCTGTCA22520.24168043021692212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21720.23309497976516644No Hit
GTACTAGTAATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGA21620.232021798458697No Hit
GCCGTATATTACTGTGCGAGACAGGATAGGAACTGGTTCGATACTTGGGG21230.22783639136346612No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19780.21227526241965897No Hit
ATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAACT19190.20594349271148915No Hit
GGATAGGAACTGGTTCGATACTTGGGGCCAGGGAATCCTGGTCACCGTCT18790.2016507674856113No Hit
TTATTAAGGTGCTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT18050.1937092258177373No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18010.19327995329514955No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16800.1802944594868691No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA16670.1788993237884588No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16210.17396268977869928No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG15830.16988460081411533No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15350.16473333054306194No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15200.16312355858335775No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTG14780.15861619709618602No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA14650.15722106139777572No Hit
CTTTTATAGTGGGAGCACCTACTATAATCCGTCCCTCAAGAGCCGAGTCA14480.15539665317677764No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14450.1550746987848368No Hit
CACCTGCACTGTCTCTGGTGGCGCCATCAGTACTAGTAATTACTACTGGG14260.15303565430254482No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14250.1529283361718979No Hit
ATATTACTGTGCGAGACAGGATAGGAACTGGTTCGATACTTGGGGCCAGG13730.1473477933782567No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13700.14702583898631585No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA13580.14573802141855252No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13490.14477215824273001No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC13090.14047943301685217No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG12930.13876234292650105No Hit
GATTATAGTAGGTGCTCCCACTATAAAAGATACTTCCAATCCACTCCAGT12750.13683061657485598No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12540.13457693583127014No Hit
GTTCCTATCCTGTCTCGCACAGTAATATACGGCCGTGTCCGCGGCGGTCA12540.13457693583127014No Hit
CCATATCAGTAGCCACGTCCAGGAACCAGTTCTCCCTGAGGCTGACCTCT12160.1304988468666862No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12120.1300695743440984No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11950.12824516612310033No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11910.12781589360051254No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA11640.12491830407304501No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11560.12405975902786943No Hit
GTCATGGACCTCCTGTGCAAGAACATCAAGCACCTGTGGTTCTTCCTCCT11460.12298657772139998No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11350.1218060782842836No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10970.11772798931969965No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGGCAGAGTCACCATCGCTT10810.1160108992293485No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10580.11354258222446875No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10490.11257671904864623No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG10480.11246940091799928No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10260.11010840204376648No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10130.10871326634535618No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9840.10560104055659476No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9800.10517176803400696No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGC9540.10238149663718636No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9470.10163026972265776No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9470.10163026972265776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14300.059.8404924
ATCAACG14550.059.2937853
CAACGCA14600.058.617195
AACGCAG15450.054.9415746
ACGCAGA18400.046.1330037
CGCAGAG19150.045.072688
GAGTCCC6650.036.7754679
TACATGG26900.036.080492
GTACATG27350.036.0493621
TGTAGCG1051.9473743E-434.23512
GTATCAA29350.029.9147971
AGGGGTG7350.029.3538325
GGGAGTC11300.029.283777
CATGGGG16450.027.976824
ATACGGC23150.027.6558236
GTAATAT23950.027.6450021
TATACGG22950.027.5759755
GGAGTCC9150.027.5136288
TAGCGCT1050.00802216827.3939674
TATCAAC31950.027.0023352
Produced by FastQC (version 0.11.5)