FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795850
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACTTGTCTTCTATGGTCAAAACAACCGGCCCTCAGGGATCCCA19560.24577495759251114No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA19070.23961801847081737No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA17810.22378588930074764No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17390.21850851291072437No Hit
GTAGTGGCAAGCGTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTG16980.21335678833951122No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAAGATCAT16950.21297983288308098No Hit
GGTATAAGCAGAAGCCAGGACAGGCCCCTATACTTGTCTTCTATGGTCAA15860.19928378463278254No Hit
AGCCAGGACAGGCCCCTATACTTGTCTTCTATGGTCAAAACAACCGGCCC15220.19124206822893763No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14770.18558773638248413No Hit
TGCCACTACTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCC14270.17930514544198028No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13500.16962995539360431No Hit
CTCTTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA13480.16937865175598416No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13320.16736822265502294No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12610.15844694351950744No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCT12500.1570647735125966No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12360.1553056480492555No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11660.1465100207325501No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG11580.14550480618206948No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCTTCACTCTTTGCATAGGTTCTG11550.14512785072563925No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11530.14487654708801911No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11150.14010177797323617No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11130.139850474335616No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10930.13733743795941447No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10900.13696048250298423No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10830.13608091977131367No Hit
GTATAGGGGCCTGTCCTGGCTTCTGCTTATACCAGTTTGCATAATAGCTT10770.13532700885845322No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10610.13331657975749198No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10590.13306527611987182No Hit
GACTATTACTGTAACTCCCGGGACAGTAGTGGCAAGCGTTGGGTGTTCGG10530.13231136520701137No Hit
CTATTATGCAAACTGGTATAAGCAGAAGCCAGGACAGGCCCCTATACTTG10450.13130615065653076No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10320.12967267701199975No Hit
GTCAAGATCATATGCCAGGGAGACAGCCTCAGAAGCTATTATGCAAACTG10240.12866746246151914No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10210.1282905070050889No Hit
GTCTGTGGCCTTGGGACAGACAGTCAAGATCATATGCCAGGGAGACAGCC10060.12640572972293776No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10020.1259031224476974No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9770.12276182697744549No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9700.12188226424577496No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9700.12188226424577496No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9670.12150530878934473No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9580.12037444242005402No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9440.11861531695671296No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT9350.11748445058742225No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCTTCACTCTTTG9080.11409185147955017No Hit
GACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACGCTTGCCA9010.11321228874787964No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8840.11107620782810831No Hit
ACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACGCTTGCCAC8720.10956838600238739No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8680.10906577872714708No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8560.10755795690142614No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT8300.10429100961236414No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8230.1034114468806936No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8200.10303449142426338No Hit
CTATTACTGTAACTCCCGGGACAGTAGTGGCAAGCGTTGGGTGTTCGGCG8100.10177797323616258No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8060.10127536596092228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)