FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990091
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCATCGACTCTCAGGCTGCTCATTTGCAGAAACA26460.2672481620376309No Hit
GTGTATTACTGTGTGAGAAACGGTGGAAGCTTCGGGGAGTCCTGGGGCCA26190.26452113997602245No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15180.15331924035265446No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14550.146956188875568No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13000.1313010622255934No Hit
GTATTACTGTGTGAGAAACGGTGGAAGCTTCGGGGAGTCCTGGGGCCAGG12950.13079605814011036No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12780.12907904424946798No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12680.12806903607850187No Hit
CTATGATACTCTCCCTCGAACTTTTGGCCAGGGGACCAAACTGGAGATCA12120.1224129903210917No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11970.12089797806464253No Hit
GTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGCCTTCGTCAAGCCTGGAG11850.11968596825948322No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11410.11524193230723237No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11310.11423192413626626No Hit
TATAAAAGGTGCCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGCCT11280.11392892168497644No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10480.10584885631724761No Hit
GCTGTGTATTACTGTGTGAGAAACGGTGGAAGCTTCGGGGAGTCCTGGGG10340.10443484487789507No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT10340.10443484487789507No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10070.10170782281628658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15300.066.474551
TCAACGC15500.065.0559544
ATCAACG15550.064.846773
CAACGCA16150.062.4344445
AACGCAG17150.058.752386
TATCAAC19200.054.0196762
CGCAGAG20500.049.1513828
GCAGAGT27050.037.5157289
GTACATG19650.031.2021181
CAGCCGT16050.030.492679
GTAATAC16400.029.908371
TACATGG21050.028.399892
ACATGGG21200.028.1989483
AATACAC18450.028.1077983
ACGCAGA36350.027.9174827
TACACAG25400.026.0869245
CATGGGA14050.025.632114
ACAGCCG29450.024.6829838
GTACACG8000.024.3076255
GTATAGG3052.1474989E-823.649821
Produced by FastQC (version 0.11.5)