FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990091
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCATCGACTCTCAGGCTGCTCATTTGCAGAAACA25740.2599761032066749No Hit
GTGTATTACTGTGTGAGAAACGGTGGAAGCTTCGGGGAGTCCTGGGGCCA23780.2401799430557393No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14230.1437241627284765No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12900.13029105405462732No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12890.1301900532375307No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12100.12221098868689848No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11880.11998897071077304No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11790.11907996335690356No Hit
GTATTACTGTGTGAGAAACGGTGGAAGCTTCGGGGAGTCCTGGGGCCAGG11110.11221190779433406No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11000.11110089880627133No Hit
GTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGCCTTCGTCAAGCCTGGAG10450.10554585386595777No Hit
CTATGATACTCTCCCTCGAACTTTTGGCCAGGGGACCAAACTGGAGATCA10320.10423284324370184No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10190.10291983262144591No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT9950.10049581301112727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC10600.054.252264
ATCAACG10750.053.4925463
CAACGCA10800.052.5899665
GTATCAA11850.052.240471
AACGCAG11800.048.142936
CGCAGAG13700.041.468278
TATCAAC14400.041.4311872
CAGCGCT5000.040.2714659
GCAGCGC6450.032.3331268
TAAGACG900.003782950331.948554
GCAGAGT18750.030.683029
GGCAGCG7450.028.9569237
GTACATG21200.028.8609091
CAGCCGT14500.027.7734229
GGATCGT1050.00802211727.3941696
TACATGG21500.026.7462732
GTAATAC15850.026.3406031
ACATGGG21550.026.0171093
GTATAGA4750.025.7621611
AATACAC17500.024.644783
Produced by FastQC (version 0.11.5)