FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874660
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGGTGCCTGCGTCGACAGGGTCCATATTGGTCATTCTAAGGACCA20500.23437678640843299No Hit
CCCCTGTACTGGTCATCTATGACGACACCAAACGACCCTCCGGGATCCCT19430.2221434614593099No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC16800.1920746347152036No Hit
GTACAGGGGCCTGGCCTGACTTCAGTTGGTACCAATAAGCATATTTTTTT14460.16532138202272884No Hit
CCTATTACTGTGCACACACCCGTGGTTCAGGCAGTTATCTGTATTTTGAC14100.16120549699311731No Hit
ATATGGACCCTGTCGACGCAGGCACCTATTACTGTGCACACACCCGTGGT9190.10506939839480484No Hit
CAGTAATAGGTGCCTGCGTCGACAGGGTCCATATTGGTCATTCTAAGGAC9140.10449774769624767No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC9130.10438341755653625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA450.00990010248.0070765
ATCAACG14400.036.507473
GTATCAA16000.036.011491
TATCAAC15200.036.0073662
AATCCGC1604.877802E-835.9991266
CAACGCA15350.034.246095
AACGCAG15850.033.160086
ACGCAGA16750.031.3765497
GTCTAAC1153.28657E-431.3143371
CGCAGAG16850.031.1885558
TACATGG24050.028.7460062
GTACATG25200.028.0089341
CTAGTAC1658.810745E-526.1871763
TCCGCTA2551.0153963E-725.4082418
CCTATAC2051.3589308E-524.5903993
GCAGAGT21250.024.3919129
TAGGTGC12850.024.0969375
ACATGGG29150.023.4696393
TAGTACC1851.916383E-423.3547944
TAATAGG13700.023.128822
Produced by FastQC (version 0.11.5)