FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113983
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACATACTCTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATG24650.22127806259161945No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20680.18564017583751277No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA19920.17881780960750748No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATAGTAC19040.17091822765697504No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.16993077991315847No Hit
GCCTACATGGAGCTGAGCATGCTGACATCTGACGACACGGCCGTTTATTT18190.16328794963657434No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCGGCAGTGACGTT17820.15996653449828227No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC17390.15610651149972665No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGCCTCATAAGT16130.14479574643419155No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16030.14389806666708557No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15990.1435389947602432No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15190.1363575566233955No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14830.13312590946181405No Hit
GTACTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGC14660.1315998538577339No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG14650.13151008588102334No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14490.13007379825365378No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14470.1298942623002326No Hit
CTCCTGCACTGGAACCGGCAGTGACGTTGGTTATTATGACTTTGTCTCCT14470.1298942623002326No Hit
GTTTAGAACCTATGAATATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14220.1276500628824677No Hit
CCTTAATATCTCTCGCACAGAAATAAACGGCCGTGTCGTCAGATGTCAGC14090.12648307918522994No Hit
GTACAATGCTGCCGCTTCTATATGAGCTGCAATAATAATCAGCCTCGTCC14030.12594447132496636No Hit
ATACCATAGAGACCCATCGTAGTTACTGTAGTCGCCTTAATATCTCTCGC13780.12370027190720144No Hit
CTTCTATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCCC13710.12307189607022727No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT13240.11885280116482927No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12950.11624952984022198No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12930.1160699938868008No Hit
CCATAGAGACCCATCGTAGTTACTGTAGTCGCCTTAATATCTCTCGCACA12910.11589045793337958No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12530.11247927481837694No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC12150.1090680917033743No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA11980.10754203609929416No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11750.10547737263495044No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11660.10466946084455508No Hit
CCATGTAGGCTGTGCCGATGGACGTGTCCCTGGTCATGGTGACCCTGCCA11470.10296386928705374No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11430.10260479738021136No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11400.10233549345007957No Hit
CTTATGAGGCACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11260.10107874177613123No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG11180.10036059796244648No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32150.059.0495071
ATCAACG34900.054.4588363
TCAACGC35250.053.918114
TATCAAC37150.051.405072
CAACGCA37400.050.6260455
AACGCAG37900.050.148116
GGTATCA14100.049.1466141
ACGCAGA40150.047.3378147
GTGCTAG5500.047.247771
CGCAGAG41150.046.36248
GAATACG5100.042.3487859
GCAGAGT48000.039.596119
GTGTAGA6300.035.5191541
TACGCAG850.002838109233.879035
GCTAGAG8150.030.033863
TGCACCG4150.029.4910815
TGTAGAG8000.027.9248352
GTACATG35950.027.9098431
GGTACCG1555.7704096E-527.868236
ACCGACC4200.027.4258798
Produced by FastQC (version 0.11.5)