FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113983
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20230.18160061688553597No Hit
ACTACATACTCTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATG18600.16696843668170877No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA17320.15547813566275248No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATAGTAC17190.15431115196551473No Hit
GTACAATGCTGCCGCTTCTATATGAGCTGCAATAATAATCAGCCTCGTCC15210.1365370925768167No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCGGCAGTGACGTT15210.1365370925768167No Hit
GCCTACATGGAGCTGAGCATGCTGACATCTGACGACACGGCCGTTTATTT14910.1338440532754988No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC14840.13321567743852464No Hit
CCTTAATATCTCTCGCACAGAAATAAACGGCCGTGTCGTCAGATGTCAGC14170.1272012229989147No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13630.12235375225654252No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGCCTCATAAGT13600.12208444832641073No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13000.11669836972377497No Hit
ATACCATAGAGACCCATCGTAGTTACTGTAGTCGCCTTAATATCTCTCGC12840.11526208209640541No Hit
GTTTAGAACCTATGAATATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12810.11499277816627362No Hit
GTACTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGC12590.1130178826786405No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12350.1108634512375862No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12070.10834994788968952No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11830.10619551644863522No Hit
GAATAGTACAATGCTGCCGCTTCTATATGAGCTGCAATAATAATCAGCCT11670.10475922882126568No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11640.10448992489113387No Hit
CTTCTATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCCC11570.1038615490541597No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11560.10377178107744912No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT11560.10377178107744912No Hit
CCATAGAGACCCATCGTAGTTACTGTAGTCGCCTTAATATCTCTCGCACA11520.10341270917060674No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG11440.10269456535692197No Hit
CTCCTGCACTGGAACCGGCAGTGACGTTGGTTATTATGACTTTGTCTCCT11360.10197642154323719No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11270.10116850975284183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG20500.041.0858463
TCAACGC20950.040.8905684
GAATACG4100.040.3835229
GTATCAA22050.040.1890531
CAACGCA21900.038.7880635
TATCAAC22500.037.7537162
AACGCAG23100.036.4614646
GGTATCA12350.035.5855871
ACGCAGA25600.032.9007767
CGCAGAG26200.031.8725578
GTGCTAG6300.029.733361
ACCGACC6000.027.5954068
CTAGTAC1856.146345E-627.2387083
TATTGAG11450.027.03485
GCAGAGT31350.026.8663399
TGCACCG6500.026.5801895
TCTACAC9300.026.31823
CCGACCG6100.025.962899
GCTAGAG7250.024.8234563
GTACATG33250.024.7015631
Produced by FastQC (version 0.11.5)