FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389565
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACACCTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20190.5182703784990951No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19400.49799134932552974No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA16380.4204689846367102No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14880.3819644988641177No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13850.3555247519669375No Hit
GTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCT12770.3278015222106709No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9920.25464299924274514No Hit
GTATATTACTGTGCGAAAGGGGGGTTTGGTTCAGGGAGTTATTATACGGT9620.24694210208822662No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9020.2315403077791896No Hit
GTGTATACCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACCC8450.21690860318560445No Hit
GGTATACACCTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAA8060.20689743688473042No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7780.19970993287384647No Hit
GGTGTATACCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACC7720.19816975344294274No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7690.1973996637274909No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7090.18199786941845392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.18020099341573295No Hit
GTGTAGAAAGACCGTATAATAACTCCCTGAACCAAACCCCCCTTTCGCAC6660.17095991683031073No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG6640.17044652368667618No Hit
TTCCATCACTATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCC6380.1637724128194268No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA6370.16351571624760952No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT6270.1609487505294367No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6260.1606920539576194No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6230.15992196424216756No Hit
TTATTACTGCATGCAAGGTATACACCTTCCGCTCACTTTCGGCGGAGGGA6200.1591518745267157No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT5850.1501674945131108No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG5760.14785722536675522No Hit
GTAGAAAGACCGTATAATAACTCCCTGAACCAAACCCCCCTTTCGCACAG5710.14657374250766883No Hit
ATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACAGCG5550.14246659735859227No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5330.13681927277861206No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5270.13527909334770832No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC5250.13476570020407377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5230.13425230706043922No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5130.13168534134226637No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT5010.12860498248045896No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA5000.1283482859086417No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT4920.12629471333410344No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA4880.1252679270468343No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4840.12424114075956516No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4780.12270096132866144No Hit
GCCGTATATTACTGTGCGAAAGGGGGGTTTGGTTCAGGGAGTTATTATAC4780.12270096132866144No Hit
GTCTTTCTACACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTC4660.11962060246685406No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA4480.11500006417414295No Hit
GCTCCTAATCTATGAAGTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGT4460.11448667103050839No Hit
CTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCC4400.1129464915996047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4390.11268979502778739No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC4330.1111496155968837No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCT4330.1111496155968837No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG4190.10755586359144173No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT4150.1065290773041726No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGCCATGAGCTGGG4110.10550229101690346No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGCC4020.10319202187054792No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA3970.10190853901146149No Hit
CCTTTAGCACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG3960.10165184243964422No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG3950.10139514586782693No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA3930.10088175272419236No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG3920.10062505615237509No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG3900.10011166300874053No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT300.001988619872.000774
GTACACG2850.042.9478265
TACACGG2900.042.2073486
GTATGTA3800.037.895141
ACACGGG3250.037.657117
CATGGGG7850.034.8538784
ACGGGGT3550.034.4748159
GGGAATT1051.929825E-434.281689
ATACGGC8050.033.987946
ATATACG8500.033.8827134
CACGGGG3650.033.53038
TACGGCC8200.033.3619277
GTAATAT9150.033.0495341
CAGCGCT1352.244306E-531.9962359
AATATAC9450.031.2384283
GGTGTAT6750.029.8669851
CTGTGCG7700.028.9836039
ACACTAT1255.3605816E-428.8003086
TATGTAC4250.028.8003082
TAATATA10350.028.5220452
Produced by FastQC (version 0.11.5)