FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389565
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACACCTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20030.5141632333500186No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18210.4674444572792731No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16100.41328148062582626No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA15810.405837280043125No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13730.3524443931051301No Hit
GTGTATACCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACCC12540.3218975010588734No Hit
GGTGTATACCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACC11250.2887836432944438No Hit
GTATATTACTGTGCGAAAGGGGGGTTTGGTTCAGGGAGTTATTATACGGT9980.2561831786736488No Hit
GTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCT9500.2438617432264192No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9030.23179700435100686No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8240.21151797517744148No Hit
GGTATACACCTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAA8170.2097210991747205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7770.1994532363020292No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7710.19791305687112548No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT7210.18507822828026133No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6940.17814742084119467No Hit
TTCCATCACTATGCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCC6910.17737733112574283No Hit
GTGTAGAAAGACCGTATAATAACTCCCTGAACCAAACCCCCCTTTCGCAC6480.1663393785375996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6440.1653125922503305No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACCCGGCTGATTTTCAGTGTGA6430.16505589567851323No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA6170.15838178481126383No Hit
TTATTACTGCATGCAAGGTATACACCTTCCGCTCACTTTCGGCGGAGGGA6090.15632821223672558No Hit
GTAGAAAGACCGTATAATAACTCCCTGAACCAAACCCCCCTTTCGCACAG5810.14914070822584163No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5690.14606034936403423No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5680.14580365279221696No Hit
ATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACAGCG5660.1452902596485824No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT5610.14400677678949597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5520.14169650764314043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5310.13630587963497748No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC5180.13296882420135278No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5130.13168534134226637No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5000.1283482859086417No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG4990.1280915893368244No Hit
GTCTTTCTACACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTC4840.12424114075956516No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4580.1175670298923158No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA4580.1175670298923158No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4580.1175670298923158No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG4580.1175670298923158No Hit
GCCGTATATTACTGTGCGAAAGGGGGGTTTGGTTCAGGGAGTTATTATAC4550.11679694017686394No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT4420.11345988474323926No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA4380.11243309845597012No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG4380.11243309845597012No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA4370.11217640188415283No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG4290.11012282930961458No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT4270.10960943616598001No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGGTTGGTACAGCCTGGGG4250.10909604302234543No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA4220.10832595330689358No Hit
GTTTATTACTGCATGCAAGGTATACACCTTCCGCTCACTTTCGGCGGAGG4040.10370541501418248No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACCCGGCTGATTTTCAGTGT4000.10267862872691334No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3950.10139514586782693No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT3920.10062505615237509No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG502.0790935E-457.597285
TCCGCTA701.7653605E-551.4261448
AATCCGC907.787318E-539.998116
CCGCTAC951.0707729E-437.8929449
CATGGGG6900.037.5634424
AAATCCG1001.4480339E-435.99835
TACACGG2501.8189894E-1234.558376
ATCCGCT1102.5350237E-432.7257237
GTATGTA4000.032.402631
GTGTTAT1153.2878332E-431.3068891
GTACACG2809.094947E-1230.8556865
GTATACC9200.029.7415453
ACACGGG2951.6370905E-1129.2867537
GTGTATA10350.029.219761
ATACGGC8400.028.2843786
ATGGGGA5350.028.2603455
TACCTTG9950.028.2197726
ATATACG8450.028.1170144
GTATATG2054.2767715E-728.0998421
TCAGTAG1306.739969E-427.6945553
Produced by FastQC (version 0.11.5)