FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014271
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC31790.3134270821111912No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC28350.2795110971328175No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAAAAGTTG26580.26206013974568926No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA26500.2612713959089829No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT26280.2591023503580404No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAAAAGTTGT25860.254961445215332No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA25740.25377832946027246No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25740.25377832946027246No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAACTTTTTTTATGTCTT25340.24983461027674064No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG24720.24372184554226634No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23910.23573581419561437No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22650.22331309876748917No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCAGTGGAACCAGCAGTGACGTT22140.2182848568084861No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20920.20625651329871406No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT19960.19679158725823767No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC19710.1943267627685303No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG19390.19117178742170485No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATAT19220.18949570676870384No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG17550.17303067917745849No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA17290.17046726170816281No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16750.16514324081039486No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16380.16149530056562791No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16320.16090374268809815No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16310.16080514970850987No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15950.15725580244333123No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGGTCCACTGCAGGTAGG15350.15134022366803349No Hit
ATGTATTACTGTGCGAGACCCCTTTATTGTAGTGGTGGTAGCTGCTCTGA15340.1512416306884452No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15090.14877680619873782No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14830.14621338872944215No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG14010.1381287644032019No Hit
GGTTATGACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAA13620.1342836381992584No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT12940.12757931558725427No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC12720.1254102700363118No Hit
CTCCTGCAGTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCT12590.12412856130166396No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12490.123142631505781No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA12420.12245248064866293No Hit
GTCAGTCACCATCTCCTGCAGTGGAACCAGCAGTGACGTTGGTGGTTATG12250.12077639999566192No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT12050.118804540403896No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11980.11811438954677793No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11900.11732564571007156No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG11570.11407207738365782No Hit
CATATGAGCTGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAG11560.11397348440406953No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGT11300.11141006693477384No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC11010.10855087052671326No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG10640.10490293028194635No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC10570.10421277942482828No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10530.1038184075064751No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC10470.10322684962894531No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG10450.10302966366976873No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10390.10243810579223896No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10220.10076202513923795No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGG10180.10036765322088476No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.059.862761
GGTATCA6050.053.512921
TCAACGC14700.052.342374
ATCAACG14900.051.6397863
TATCAAC15950.049.6026342
CAACGCA16300.046.756385
AACGCAG17000.045.2160876
CGCAGAG19000.040.183498
ACGCAGA20650.036.9727027
GCAGAGT23200.032.598439
TCTACAC9350.032.3016433
CAGAGTA22150.029.5912349
GTACATG26500.028.5065671
GTATATA4350.028.1164931
ACATGGG26950.028.0167313
GGGCAGT6950.026.914731
GGATATT7050.026.532961
TATTGGG9000.026.3720682
TACCGCA1100.009985127526.191647
ATTGGGG9650.025.336033
Produced by FastQC (version 0.11.5)