FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188805
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG49650.41764629186451946No Hit
GTAATAGACGGCCGTGTCATCAGATCTCAGGCTGTTCAGCTCCATGTAGG48190.4053650514592385No Hit
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTTGGGTGTTCGGCGG45390.3818119876682887No Hit
GTCTATTACTGTGCGAGGCCACGTATAGTGGGACCTACTAGGGGGACCAA41770.35136124090998944No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC37890.3187234239425305No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCGCCAGG37210.31300339416472844No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC34690.2918056367528737No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32430.2727949495501785No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACTCAGACTG29320.24663422512523078No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26420.2222399804846043No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26380.22190350814473356No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26340.2215670358048629No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT25890.21778172198131737No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25340.2131552273080951No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24730.20802402412506676No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA24410.20533224540610107No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24230.20381811987668288No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23690.19927574328842831No Hit
CTACTAGGGGGACCAACTACGGCATGGACGTCTGGGGCCAAGGGACCACG23640.19885515286358993No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23470.1974251454191394No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22980.19330335925572317No Hit
GCCTACATGGAGCTGAACAGCCTGAGATCTGATGACACGGCCGTCTATTA22530.18951804543217768No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22500.18926569117727465No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22360.18808803798772716No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21750.18295683480469882No Hit
TCCCTATCTTTGATACAAGAAACTACGCACAGAAGTTCCAGGGCAGAGTC20890.17572267949747855No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGATCAC20790.17488149864780178No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20210.17000264971967646No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20200.1699185316347088No Hit
ACCATGGACTGGCCCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG19990.1681520518503876No Hit
CCCTAGTAGGTCCCACTATACGTGGCCTCGCACAGTAATAGACGGCCGTG19930.1676473433405815No Hit
GTCTGGGGCTGAAGTGAAGCCGCCTGGGTCCTCGGTGAAGGTCTCCTGCA19910.16747910717064615No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG19650.16529203696148653No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19410.16327320292226227No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA19100.16066554228826427No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18740.15763729122942788No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA18580.15629140186994503No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18520.15578669336013895No Hit
GATTATTACTATAGATGAGGAGTTGGGGGGCCGTTCCGGGGAGTTGCTGG17910.15065549017711063No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17330.14577664124898534No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16710.1405613199809893No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16620.1398042572162802No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16580.1394677848764095No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT16540.1391313125365388No Hit
GCTCATCACCATGGACTGGCCCTGGAGGTTCCTCTTTGTGGTGGCAGCAG16330.13736483275221756No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT16300.13711247849731453No Hit
CATAACAACCACATTCCTCCTCTGAAGAAGCCCCTGGGAGCACAGCTCAT16070.13517776254305794No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15940.13408422743847814No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15840.13324304658880137No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACTCAGACTGT15720.13223362956918924No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15460.1300465593600296No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15350.12912126042538516No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15210.12794360723583767No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT15170.12760713489596698No Hit
CATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG14250.11986827107894063No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14170.1191953263991992No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG14140.11894297214429617No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14030.11801767320965172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.11549413066062138No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG13720.1154100125756537No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC13700.11524177640571834No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13660.11490530406584763No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA13650.11482118598087995No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGAT13500.1135594147063648No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13440.11305470619655873No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC13400.11271823385668803No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG12980.10918527428804556No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12930.10876468386320716No Hit
CGTATAGTGGGACCTACTAGGGGGACCAACTACGGCATGGACGTCTGGGG12910.10859644769327181No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12810.10775526684359503No Hit
TTATTACTGTGCAGCATGGGATGACAGTCTGAGTTGGGTGTTCGGCGGAG12590.10590466897430614No Hit
GAGCTGAACAGCCTGAGATCTGATGACACGGCCGTCTATTACTGTGCGAG12580.10582055088933845No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12440.10464289769979097No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12440.10464289769979097No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12410.10439054344488793No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12400.10430642535992027No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA12200.1026240636605667No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12200.1026240636605667No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12090.10169876472592226No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12070.10153052855598689No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11970.10068934770631013No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC11910.10018463919650406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.085.175371
TCAACGC23850.070.724684
ATCAACG23950.070.12843
TATCAAC24500.068.848312
CAACGCA24950.067.317645
AACGCAG26300.063.778896
CGCAGAG30300.055.112378
ACGCAGA36000.046.3862467
GAATACG2900.044.6764039
CAGCGCT5950.042.3403859
GTATCAA42450.041.7911071
GTGCTAG3650.041.4909021
GCAGCGC7450.036.7139438
TAATACG800.00209500336.0423854
GTACATG29300.033.7193681
TATTGAG17000.032.650165
ACATGGG30450.032.1954963
GGCAGCG8650.031.6206827
TGCTAGA5400.030.7027722
AGAATAC4750.030.3068038
Produced by FastQC (version 0.11.5)