FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188805
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCATCAGATCTCAGGCTGTTCAGCTCCATGTAGG46000.3869431908513171No Hit
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTTGGGTGTTCGGCGG43830.36868956641333106No Hit
GTCTATTACTGTGCGAGGCCACGTATAGTGGGACCTACTAGGGGGACCAA40090.33722940263541956No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCGCCAGG36180.30433923141305763No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC36040.30316157822351014No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35660.29996509099473845No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34070.2865903154848777No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28730.2416712581121378No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG28120.23654005492910948No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24810.20869696880480818No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23620.19868691669365454No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACTCAGACTG23600.19851868052371918No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23560.19818220818384852No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT23310.19607925605965656No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23220.19532219329494743No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22140.18623744011843826No Hit
GCCTACATGGAGCTGAACAGCCTGAGATCTGATGACACGGCCGTCTATTA21690.18245212629489277No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21640.18203153587005438No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21570.18144270927528064No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21480.18068564651057153No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21450.1804332922556685No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA20980.17647974226218766No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20750.17454502630793106No Hit
CTACTAGGGGGACCAACTACGGCATGGACGTCTGGGGCCAAGGGACCACG20390.17151677524909467No Hit
GATTATTACTATAGATGAGGAGTTGGGGGGCCGTTCCGGGGAGTTGCTGG20390.17151677524909467No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGATCAC20330.1710120667392886No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19420.16335732100722997No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18710.15738493697452483No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18660.15696434654968644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18100.1522537337914965No Hit
CCCTAGTAGGTCCCACTATACGTGGCCTCGCACAGTAATAGACGGCCGTG18030.15166490719672276No Hit
GTCTGGGGCTGAAGTGAAGCCGCCTGGGTCCTCGGTGAAGGTCTCCTGCA18000.15141255294181974No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA17920.1507396082620783No Hit
ACCATGGACTGGCCCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG17490.14712253060846817No Hit
TCCCTATCTTTGATACAAGAAACTACGCACAGAAGTTCCAGGGCAGAGTC17150.14426251571956714No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA17140.14417839763459947No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16810.14140250083066608No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA16430.13820601360189433No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG16270.1368601242424115No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT16230.1365236519025408No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16200.13627129764763773No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15850.13332716467376904No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15490.13029891361493265No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15230.12811184340577303No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT14920.12550418277177502No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14860.12499947426196895No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14780.12432652958222752No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14750.12407417532732451No Hit
CATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG14610.122896522137777No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14230.11970003490900526No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14020.11793355512468404No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13800.11608295725539512No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13670.11498942215081533No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13630.11465294981094461No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT13530.11381176896126782No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13360.11238176151681732No Hit
GCTCATCACCATGGACTGGCCCTGGAGGTTCCTCTTTGTGGTGGCAGCAG13360.11238176151681732No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC13300.11187705300701124No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG13180.11086763598739911No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13180.11086763598739911No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACTCAGACTGT13120.11036292747759303No Hit
CATAACAACCACATTCCTCCTCTGAAGAAGCCCCTGGGAGCACAGCTCAT13070.10994233705275466No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGAT12380.1041381891899849No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12220.10279229983050206No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC12090.10169876472592226No Hit
CTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT12040.10127817430108385No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG11900.10010052111153638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)