FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097447
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACA47700.4346451354826247No Hit
GTGTATTACTGTGCGAGAGATCTAATACCAGTGCCTGATAGCGGTGACTA47440.43227600057223725No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG31910.290765749963324No Hit
ATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT31290.28511627440778464No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29570.2694435357698367No Hit
ATCTAATACCAGTGCCTGATAGCGGTGACTACTGGGGCCAGGGAACCCTG26980.24584330723943842No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26670.24301856946166875No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT26570.24210736372690436No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26060.23746021447960583No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25980.23673124989179434No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25200.22962384516063192No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23820.217049206020883No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAATGGGTGGC23000.2095773189958148No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22590.20584137548328077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20330.18524812587760503No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19510.17777623885253682No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG19290.17577158623605516No Hit
GTACTAAATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGA19270.17558934508910226No Hit
ACTGTAAACAGTATAGTTTTCATTGGACGTTCGGCCAAGGGACCAAGGTG18920.17240012501742683No Hit
GTATTAGATCTCTCGCACAGTAATACACAGCCGTGTCCTCAGGTCTCAGG18000.1640170322575942No Hit
TCCATAAGGCGTCCAGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGC17990.16392591168411777No Hit
GTATTACTGTGCGAGAGATCTAATACCAGTGCCTGATAGCGGTGACTACT17470.15918764186334283No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16920.15417601032213857No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16620.15144239311784533No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA16570.1509867902504631No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16140.14706860559097615No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15160.1381387893902849No Hit
GAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGAAGCGCCC14660.13358276071646283No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG14580.1328537961286513No Hit
CAGTTATATCATATGATATAAGTACTAAATATTACGCAGACTCCGTGGAG14350.13075802293869315No Hit
GCGTAATATTTAGTACTTATATCATATGATATAACTGCCACCCATTCCAG14320.13048466121826385No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG14230.12966457605697587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14080.12829776745482924No Hit
GCCTGAGACCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATCTAATA13940.1270220794261591No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT13820.12592863254444178No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCCGAGCTGGGTTTTCCTCGT13620.12410622107491295No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT13600.12392397992796007No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA13530.12328613591362499No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13460.1226482918992899No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13440.12246605075233702No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCACTAGCTATGCTATGCACTGGG13280.12100812157671396No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT13100.11936795125413802No Hit
GTTTACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTG13090.11927683068066158No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12810.11672545462332122No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCCGAGCTGGGTTTTC12800.11663433404984479No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAA12800.11663433404984479No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12790.11654321347636834No Hit
TCTAATACCAGTGCCTGATAGCGGTGACTACTGGGGCCAGGGAACCCTGG12620.11499416372726884No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12550.11435631971293374No Hit
GCTGTGTATTACTGTGCGAGAGATCTAATACCAGTGCCTGATAGCGGTGA12520.11408295799250442No Hit
ATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACAGCG12500.11390071684555154No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12440.1133539934046929No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC12320.1122605465229756No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12280.11189606422906982No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12130.11052925562692321No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCCGAGCTGGG12080.11007365275954101No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11900.10843348243696506No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC11720.10679331211438912No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11630.10597322695310116No Hit
CAGTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATA11620.10588210637962471No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11490.10469753892443097No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11480.10460641835095455No Hit
ATTTAGTACTTATATCATATGATATAACTGCCACCCATTCCAGCCCCTTG11470.1045152977774781No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT11430.10415081548357233No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG11140.10150831885275553No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCACTAGCTATGCT11110.10123495713232619No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAATGGGTG11090.10105271598537333No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10990.10014151025060893No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)