FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097447
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACA43930.40029267928200635No Hit
GTGTATTACTGTGCGAGAGATCTAATACCAGTGCCTGATAGCGGTGACTA43520.39655673576947226No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG27560.251128300501072No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27150.24739235698853795No Hit
ATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT26090.23773357620003516No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25070.22843927770543818No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23680.21577351799221284No Hit
ATCTAATACCAGTGCCTGATAGCGGTGACTACTGGGGCCAGGGAACCCTG23610.21513567397787775No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT22840.20811938982019176No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22550.20547689318937498No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22050.2009208645155529No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21030.1916265660209559No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20720.18880182824318623No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAATGGGTGGC19240.17531598336867293No Hit
GTACTAAATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGA18500.16857306093141627No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18460.16820857863751051No Hit
GTATTAGATCTCTCGCACAGTAATACACAGCCGTGTCCTCAGGTCTCAGG17950.163561429390212No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG17440.1589142801429135No Hit
ACTGTAAACAGTATAGTTTTCATTGGACGTTCGGCCAAGGGACCAAGGTG17170.1564540246590496No Hit
GTATTACTGTGCGAGAGATCTAATACCAGTGCCTGATAGCGGTGACTACT16820.15326480458737415No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16570.1509867902504631No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15920.14506395297449445No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15720.14324154150496562No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15470.1409635271680546No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15090.1375009453759498No Hit
TCCATAAGGCGTCCAGTTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGC15030.13695422193509116No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14360.1308491435121696No Hit
GTTTACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTG14160.12902673204264078No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14080.12829776745482924No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14050.12802440573439994No Hit
GCGTAATATTTAGTACTTATATCATATGATATAACTGCCACCCATTCCAG13760.12538190910358316No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG13250.12073475985628464No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT12730.11599649003550967No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12680.11554088716812748No Hit
CAGTTATATCATATGATATAAGTACTAAATATTACGCAGACTCCGTGGAG12560.11444744028641018No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12430.11326287283121646No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCACTAGCTATGCTATGCACTGGG12200.1111670996412583No Hit
GAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGAAGCGCCC12180.11098485849430542No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11960.10898020587782373No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11910.1085246030104415No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCCGAGCTGGGTTTTCCTCGT11890.10834236186348861No Hit
GCCTGAGACCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATCTAATA11880.10825124129001218No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11850.10797787956958287No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT11630.10597322695310116No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11470.1045152977774781No Hit
GCTGTGTATTACTGTGCGAGAGATCTAATACCAGTGCCTGATAGCGGTGA11440.10424193605704876No Hit
CAGTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATA11390.10378633318966657No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC11320.10314848917533147No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC11070.10087047483842045No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11020.10041487197103822No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)