FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211487
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTA40920.33776672799625584No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33020.27255760895494546No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT32430.2676875608240121No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22430.18514437216412558No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20980.17317560980844202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20840.17202000516720362No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG20430.16863573443214827No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19530.16120684745275846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18540.1530350717754297No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC16940.13982816158984784No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16800.13867255694860944No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT15370.12686888097024565No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15150.12505293081972815No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT14490.11960508036817563No Hit
CAGTATAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGAT14150.1167986119537395No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC12920.10664579974857344No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG12150.10028997422176218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG6750.042.660069
TCAACGC46200.041.6039474
ATCAACG46450.041.535013
TATCAAC47300.040.8408662
CAACGCA47600.040.6827775
GTGCTAG8000.040.6177861
GTATCAA49400.039.9051931
AACGCAG49300.039.4259456
CGCAGAG51400.037.815168
ACGCAGA52400.036.818737
CTAGAGA8950.035.391174
GCAGAGT56850.034.1899579
TAGGTGG12500.033.978745
CCGATCG850.002838994433.877119
GTCATAG13650.033.3274151
TATACTG18800.033.313995
GCTAGAG8700.033.0983243
GTGTAGA7800.032.4016531
TTATACT19550.031.6677344
ATACTGT21750.031.4434076
Produced by FastQC (version 0.11.5)