FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211487
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTA34020.2808119278209341No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT27020.22303169575901352No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24300.20057994844352436No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22950.1894366179744397No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG19090.15757494715172346No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16930.13974561840118796No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16720.13801221143933035No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC14870.12274172153725132No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14350.11844947572693723No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14020.11572555050116096No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT13830.11415722991662312No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12670.10458222003207629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTGA5800.047.1636289
CCCCGTT7000.039.0784347
GTCATAG12750.037.3005941
CCCGTTG7400.035.9932948
ACCCCGT7850.035.7640386
TCAACGC31650.034.571764
GAATACG6450.033.4821369
ATCAACG32050.033.2418563
TCATAGG14100.032.164222
TATCAAC34700.031.7404822
CAGAGCG14550.031.6642029
ATCTCAC9350.031.5976091
GTATCAA38000.031.4779531
CAACGCA35050.031.2181515
ACGCAGA35500.030.4168687
TAGGTGG13500.030.3943375
AACGCAG36200.030.2264126
CGCAGAG35750.030.0028028
ATAGGTG13250.029.8812264
GTGCTAG8350.029.3409651
Produced by FastQC (version 0.11.5)