FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047184
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG40920.39076227291478866No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG40390.3857010802304084No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT30460.29087533804947363No Hit
ATAATACACGGCCGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACA28820.27521428898837264No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG28810.27511879478678053No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG28770.2747368179804122No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG25040.23911748078656667No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT24520.2341517823037785No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21970.20980076089779828No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC21720.2074134058579963No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT21240.2028296841815765No Hit
CAATAATACACGGCCGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATA19920.19022444957142204No Hit
CCCAATACTTCTAGCACAATAATACACGGCCGTGTCCTCGATTTTCAGGC19830.1893650017570933No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGGAGG17070.16300860211767942No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16610.15861586884444376No Hit
ATACACGGCCGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAGTG16410.1567059848126022No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14710.14047197054194868No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT14450.13798912130055463No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC14340.13693868508304177No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT14200.13560176626075265No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGG14170.1353152836559764No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC14060.13426484743846354No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14050.13416935323687146No Hit
GACCTAGGACGGTCAGCTTGGTGCCTCCGCCGAACACCAGAGTTCTGCTC13600.1298721141652279No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGACTGCACAGGAGAGTC13480.12872618374612294No Hit
GTATTATTGTGCTAGAAGTATTGGGAGCGCAACCTCCTTTGACTCCTGGG13240.12643432290791304No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG13130.12538388669040015No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC13060.1247154272792556No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGACGTT12780.12204158963467739No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.11707589115188925No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC11900.11363809989457441No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGAG11850.113160628886614No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG11740.11211019266910112No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC11730.11201469846750907No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11730.11201469846750907No Hit
GTGTATTATTGTGCTAGAAGTATTGGGAGCGCAACCTCCTTTGACTCCTG11340.10829042460541796No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11280.10771745939586548No Hit
GTGTAGCTGTTCGCTTTGTTTCTAATACGGCCAACCCACTCCAGCCCCTT11230.10723998838790508No Hit
GTCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGC11230.10723998838790508No Hit
GATTATTACTGCACCTCATATACAAGGAGCAGAACTCTGGTGTTCGGCGG11050.10552109275924766No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG10860.10370670292899815No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT10710.10227428990511696No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC10530.10055539427645953No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA10520.10045990007486745No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG701.7681194E-551.422745
CCGTTGA6400.043.870029
CCCGTTG6800.041.2894338
CAGAGCG15800.040.5523649
CCCCGTT7400.037.9416437
ACCCCGT7400.037.9416436
GCACACA17700.035.83192
GTCATAG15950.035.7444421
TAGGTGG15750.035.65315
ATAGGTG15950.034.754684
ACAGAGC18600.034.4477048
ATCAACG39950.032.6170773
TCAACGC39950.032.6170774
CAACGCA40000.032.576315
AACGCAG41100.031.5292686
TATCAAC41700.031.109782
ATCTCAC9250.030.4274121
CATGGGG16550.030.0147234
CATAGGT18950.030.0124243
CGCAGAG44100.029.3844228
Produced by FastQC (version 0.11.5)