FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047184
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39950.3814993353603569No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG32110.30663188131216673No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG27810.26556937462757263No Hit
ATAATACACGGCCGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACA23730.22660774037800424No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG23190.22145105349203198No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20740.19805497410197254No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC19440.18564072789500222No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18600.17761921496126756No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17410.1662554049718101No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16600.1585203746428517No Hit
CAATAATACACGGCCGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATA16340.15603752540145763No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16050.1532681935552873No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15730.1502123791043408No Hit
CCCAATACTTCTAGCACAATAATACACGGCCGTGTCCTCGATTTTCAGGC14700.1403764763403566No Hit
ATACACGGCCGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAGTG13890.13264144601139818No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13040.12452443887607145No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGGAGG13010.1242379562712952No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT12680.12108664761875658No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGG12320.11764885636144173No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGACTGCACAGGAGAGTC12240.11688490274870511No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11870.11335161728979817No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC11390.10876789561337837No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA11270.10762196519427339No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC11250.10743097679108925No Hit
GTATTATTGTGCTAGAAGTATTGGGAGCGCAACCTCCTTTGACTCCTGGG11130.10628504637198428No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT11080.10580757536402388No Hit
GACCTAGGACGGTCAGCTTGGTGCCTCCGCCGAACACCAGAGTTCTGCTC10510.10036440587327539No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)