FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156995
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA42870.3705288268315766No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT38670.3342278920825068No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAATTACTATTGGAGCTGGATCCG34350.2968897877691779No Hit
GTATTATTGTCAGCTCTATGGTACTACGCCTTGGTACACCTTTGGCCAGG30380.2625767613516048No Hit
TCTATGAAGTATCCACGAGGGCCTCTGGCATCCCAGACAGGTTCACTGGC30110.26024312983202175No Hit
GTGTATTACTGTGCGAGAGACTTCACCTATGGGACACATTTTCATTCTTG26300.22731299616679415No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA25740.22247287153358483No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT25670.2218678559544337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23880.2063967432875682No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT23000.19879083314966792No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT22600.1953336012688041No Hit
CTATGGTACTACGCCTTGGTACACCTTTGGCCAGGGGACCAAGCTGGAGA21650.1871226755517526No Hit
CAATAATACACTGCAAAGTCTTCAGGCTCCAGTCTGTTGATGGTGAGAGT20210.17467664078064296No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC19820.17130583969680077No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19680.17009580853849846No Hit
TATCTATACCACTGGGAGCACCAATTACAACCCTTCCCTCAAGAGTCGAG19520.16871291578615294No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19180.1657742686874187No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18300.15816835854951836No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG18250.1577362045644104No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18220.1574769121733456No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT17330.14978457123842367No Hit
GTACTACGCCTTGGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAA16070.13889429081370275No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16060.13880786001668116No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG15930.13768425965540043No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCT15760.1362149361060333No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAATTACTATTGGA15490.13388130458645023No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15260.13189339625495358No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15260.13189339625495358No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14760.12757185640387383No Hit
GTACCATAGAGCTGACAATAATACACTGCAAAGTCTTCAGGCTCCAGTCT14710.12713970241876585No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14330.12385533213194524No Hit
ATCTATACCACTGGGAGCACCAATTACAACCCTTCCCTCAAGAGTCGAGT13970.12074382343916785No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA13910.12022523865703827No Hit
CTTCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAA13830.11953379228086551No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT13740.11875591510767117No Hit
CTCCTGCAGGGCCAGTCAGAGTTTTAGAGGCAACTTCTTAAGTTGGTACC13610.11763231474639044No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13530.11694086837021767No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13440.11616299119702332No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13430.11607656040000174No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13030.11261932851913793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12840.11097714337572764No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12590.10881637345018778No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA12500.10803849627699341No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC12270.10605058794549674No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12240.10579129555443195No Hit
CTATACCACTGGGAGCACCAATTACAACCCTTCCCTCAAGAGTCGAGTCA12220.10561843396038877No Hit
ATAATACACTGCAAAGTCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGA12200.10544557236634557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.10492698758421601No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA12070.10432197200506484No Hit
GCTCTATGGTACTACGCCTTGGTACACCTTTGGCCAGGGGACCAAGCTGG11900.10285264845569773No Hit
TCAGTAGACATGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCTGTGAC11840.10233406367356818No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC11730.10138332490633062No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)