FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862939
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21580.25007561368764186No Hit
TCCCAGGAACGGCCCCCAAAGTCCTCATCTATTTTGATCATCAGCGGTCC19660.2278260688183058No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAACCATCCAGG17320.20070943600880245No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16920.1960741141610241No Hit
ATATTATTGTGCGAGAGACTTTAAGCGCCCGGGAGGCTTCGGGGGTAACA16490.19109114317466241No Hit
ATAATATATGGCAGTATCTTCAGGCCTTAGACTGGTGATCTGCAGATATG16170.18738288569643974No Hit
GAAATAATCACCCTCATCTTCAGACTGGAGGTCACCGATGGCCAGGGAGG14740.17081161009063212No Hit
GTCCTGGGCCCAGTCTATGTTGACTCAGCCACCGTCAGTGTCTGGGACCC14420.16710335261240944No Hit
ATATATTATTGTGCGAGAGACTTTAAGCGCCCGGGAGGCTTCGGGGGTAA14130.16374274427277014No Hit
TTCCTGGGATGTGCTGATACCAGTTAACAATGTTACTGCCGATGTTTGAG13770.15957095460976964No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13640.15806447500924167No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13300.15412445143863007No Hit
GCCATATATTATTGTGCGAGAGACTTTAAGCGCCCGGGAGGCTTCGGGGG13170.15261797183810213No Hit
CAATAATATATGGCAGTATCTTCAGGCCTTAGACTGGTGATCTGCAGATA13030.1509956091913797No Hit
GTGCAGGGTCCTGGGCCCAGTCTATGTTGACTCAGCCACCGTCAGTGTCT12970.15030031091421292No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA12910.1496050126370462No Hit
ATATATGGCAGTATCTTCAGGCCTTAGACTGGTGATCTGCAGATATGCCG11950.13848024020237815No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11700.1355831640475167No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11330.13129549133832172No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11180.12955724564540483No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10680.12376309333568189No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10550.12225661373515392No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTATGTTGACTCAGCCAC10500.12167719850418163No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10470.12132954936559827No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10400.12051836804223705No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10260.11889600539551463No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG10200.11820070711834788No Hit
CCTCTGGCACCACGGCATATCTGCAGATCACCAGTCTAAGGCCTGAAGAT9790.11344950222437508No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9680.11217478871623603No Hit
GTCTAAGGCCTGAAGATACTGCCATATATTATTGTGCGAGAGACTTTAAG9650.11182713957765264No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9370.1085824142842078No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9110.1055694550831519No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8890.10302002806687378No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8870.10278826197448487No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8830.10232472978970704No Hit
GTCTATGTTGACTCAGCCACCGTCAGTGTCTGGGACCCCCGGGCAGGGGG8770.10162943151254028No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAACCATCCAGGC8750.10139766542015137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)