FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144312
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGGTCCGTTCACTGTTGGCCAGGGGACCAAGCTGGAGATCA81570.7128300673242962No Hit
CTATAATATTGTAAACAGAAGTAAACTGCCACATCTTCAGCCTGCAGGCT52330.457305350289082No Hit
GTAATAGACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA43480.37996630289641287No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35350.30891924580009644No Hit
ATTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA33750.29493704514153485No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29250.2556121057893302No Hit
GTCTATTACTGTGCGAAAGTCCTCGATTGTAATCTTACCACCTGCACAGA23640.2065870147302484No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18860.16481519026279545No Hit
ATAGTGGTCCGTTCACTGTTGGCCAGGGGACCAAGCTGGAGATCAAACGA18250.15948447626171883No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16110.14078328288089262No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15490.13536518012569998No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14440.1261893609435189No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14160.12374247582827061No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14010.12243164451653046No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13690.11963520438481813No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12860.11238193779318927No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12800.11185760526849321No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12500.10923594264501289No Hit
GTCATGGAACCATGGGGAGACCTTCACCTGCACTGCTGCCCACCCCGAGT12480.1090611651367809No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12430.10862422136620083No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12040.10521605995567641No Hit
GACCACTATAATATTGTAAACAGAAGTAAACTGCCACATCTTCAGCCTGC11930.10425478366040031No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11920.1041673949062843No Hit
GGACAGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT11620.10154573228280399No Hit
GTTCACTGTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG11460.10014751221694783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)