FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247681
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTTCTCAGCTCCATGTAGG18790.1505993919920236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.12270764722713577No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14910.11950169955301075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT350.00366598661.713676
TCAACGC27650.060.6745154
GTATCAA29850.059.8496781
ATCAACG28250.059.64793
CAACGCA29050.057.5002825
AACGCAG29750.056.3893556
GGTATCA10400.054.720271
TATCAAC31950.052.7403222
CGCAGAG32800.051.141738
ACGCAGA37100.045.214257
GCAGAGT51300.033.260169
TTTCGCG1352.2478245E-531.9971149
GTACACG5100.031.058515
ATAGACG8150.030.9211754
GTACATG32300.029.4391941
TAGACGG8500.028.7997115
TAGTACG2054.291469E-728.0984064
GTAATAG11400.027.803681
TACACGG5950.026.6215826
AGACGGC9200.026.6084296
Produced by FastQC (version 0.11.5)