FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900688
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23030.25569342547030716No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTTAGG18370.20395519869255502No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT15670.17397811450802053No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC15230.1690929600483186No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA15040.16698346153162916No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14420.16009983479295828No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14290.1586564937025918No Hit
GAAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG13870.15399339171833087No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13720.1523279981525234No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13360.14833105359458548No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13090.14533334517613203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.1404481907164301No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12570.13955998081466614No Hit
TATGTATACTGGTACCAGAGGGTCACAGGAATGGCCCCCAAACTCCTCAT12350.13711740358481517No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11430.12690298971452935No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11240.12479349119783986No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10840.12035244168901996No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10510.1166885758442435No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10450.11602241841792052No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10390.11535626099159753No Hit
GATTATTTCTGTGCAACATGGGATGACAGCCTAAGTGGTTGGGTGTTCGG10240.11369086742579007No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTTAGGC10220.11346881495034908No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10130.11246957881086458No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9990.11091521148277761No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9980.11080418524505713No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT9920.11013802781873414No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9650.10714031940028067No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9650.10714031940028067No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9460.10503082088359121No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCAGATAGG9400.10436466345726822No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9320.10347645355550425No Hit
GGTACCAGAGGGTCACAGGAATGGCCCCCAAACTCCTCATCTCTCGGAAT9150.10158900751425577No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9120.10125592880109427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8000.064.8886261
GTATCAA19500.062.4853441
ATCAACG20900.057.5553673
TCAACGC21250.056.6073954
CAACGCA21550.055.819365
TATCAAC22300.053.9420242
AACGCAG22600.052.857336
ACGCAGA24700.048.3633887
CGCAGAG25600.046.9442148
GCAGAGT30100.040.6432089
GAATACG2603.6379788E-1233.213419
GTACATG23900.027.4517211
GTGCTAG3950.027.379171
TACATGG23400.027.0883432
TACACGG6950.025.9102045
CAGAGTA29900.025.511759
GTATAAC2851.0495569E-825.297711
TATTGAG12300.025.1813495
ATACACG6750.024.543684
TCTACAC7700.023.3864823
Produced by FastQC (version 0.11.5)