FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900688
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC22450.24925390368251824No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC18260.20273391007762953No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT17080.18963281402661075No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTTAGG16480.18297123976338087No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13950.15488160162009484No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA13450.14933028973406995No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13200.14655463379105751No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12750.14155845309363507No Hit
TATGTATACTGGTACCAGAGGGTCACAGGAATGGCCCCCAAACTCCTCAT12720.14122537438047358No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12450.13822766596202016No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12310.13667329863393315No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12150.13489687883040521No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT11520.1279022258540138No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11410.12668093723908833No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11230.12468246496011937No Hit
GATTATTTCTGTGCAACATGGGATGACAGCCTAAGTGGTTGGGTGTTCGG10280.11413497237667207No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10000.1110262377204981No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9960.1105821327696161No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9760.10836160801520615No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9580.1063631357362372No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9440.10480876840815022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12750.054.7122544
GTATCAA12900.054.68221
ATCAACG13000.053.6600953
CAACGCA13750.050.2157445
AACGCAG14050.049.658196
TATCAAC14600.047.776882
ACGCAGA15550.044.865527
CGCAGAG16850.041.4109958
CGATAGT700.001099501241.0946244
GGTATCA6500.034.3287471
GCAGAGT20700.034.056469
GTACATG25350.033.2213671
TACATGG24950.032.2809562
GAATACG2757.2759576E-1231.3900769
TATTGAG14900.029.445235
TTATTGA16600.027.2932664
GTCTAGA3501.4188117E-1024.678731
CTAGGTC17550.024.176753
CCTAGGT18000.023.970532
GTGCTAG4600.023.4716171
Produced by FastQC (version 0.11.5)