FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666607
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGCTCTCAGGCTGGTCATTTGCAGAAACA26900.40353611648242516No Hit
GTCTATTACTGTGTGACAGACTCCGATGGCTACGATTTCAACTTTCACTA21870.32807936310299773No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11000.16501476882180957No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG10990.1648647553956079No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10840.16261455400258323No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10450.15676403038071907No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9440.14161267433435293No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8890.13336193589326245No Hit
GATAAATACTACGCAGACTCCGTGGAGAGCCGATTCACCATCTCCAGAGA8820.13231184190985093No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8550.1282614794024065No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8460.12691135856659172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.12601127800938183No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8060.12091082151852592No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7580.11371017706084693No Hit
GTATTTATCACTTCCATCATATGATATAACTGCCACCCACTCCAGTCCCT7130.10695957288177292No Hit
ATACTACGCAGACTCCGTGGAGAGCCGATTCACCATCTCCAGAGACAATT6810.10215914324332029No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCGAGGGACTGGAGTGGGTGGC6670.10005895527649726No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA450.00990720947.99719
GTATCAA23800.037.518781
TCAACGC23700.036.1497154
ATCAACG23900.035.8472063
CAACGCA24200.035.4028175
CAGCCGT9600.035.247879
AACGCAG24750.034.616096
TAGACAG10900.033.686045
TATCAAC25450.033.0981982
CGCAGAG26050.032.8886078
GTAATAG11500.031.3095021
ATAGACA11450.030.8103664
GCAGAGT28700.030.6044229
CAGCGCT3300.030.543619
GTCTATT13600.029.122441
AATAGAC11850.028.5552393
TAATAGA12750.028.2335872
TCTATTA14550.027.7096632
TACACGG5750.027.5461626
ACTGTGT16900.026.8386158
Produced by FastQC (version 0.11.5)