FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666607
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGCTCTCAGGCTGGTCATTTGCAGAAACA27810.41718733826677484No Hit
GTCTATTACTGTGTGACAGACTCCGATGGCTACGATTTCAACTTTCACTA21900.32852940338160264No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13850.20776859528927838No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG10780.16171447344537335No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10090.15136354703745986No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10000.15001342620164504No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9230.1384623923841184No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8390.1258612645831802No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8230.12346104976395386No Hit
GTATTTATCACTTCCATCATATGATATAACTGCCACCCACTCCAGTCCCT8200.12301100948534895No Hit
GATAAATACTACGCAGACTCCGTGGAGAGCCGATTCACCATCTCCAGAGA8060.12091082151852592No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7740.11611039188007327No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7250.10875973399619265No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6990.1048593849149499No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6880.1032092372267318No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6840.1026091835219252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)