FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018781
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGGCTGGATCCGGCA71160.6984818130687558No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAAA55660.5463392034205584No Hit
GTCTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATTC48400.47507756819179003No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28730.28200368872211007No Hit
CTACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAG28420.2789608365291461No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27600.2709120016961447No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27290.26786914950318075No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26130.25648299291015436No Hit
ACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACACGTCG25450.24980834939010446No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25140.2467654971971405No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24530.24077794933356628No Hit
GTATTACTGTCAGCAATATGATGACTCACCCACTTTCGGCGGAGGGACCA24290.23842219279707808No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23980.23537934060411414No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21670.21270518394041507No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21290.20897523609097537No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19280.1892457750978866No Hit
AACCAGTAGTGGGCCGGGGAATAACCACTCCAAAAATCGTAATATCTCGC18570.1822766620107756No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17230.1691236880153831No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACAC17000.16686608800124855No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16700.16392139233063827No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16390.16087854013767433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16070.15773753142235672No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15680.15390942705056337No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15410.15125920094701414No Hit
CACTTGCACTGTCTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGGCT15320.15037579224583106No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA14730.1445845574269642No Hit
GTAGTAGGTGCTCCCACCATGAAAGATACTTGCAATCCACTCCGGCCCCT14570.1430140530693054No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG14480.1421306443681223No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14320.1405601400104635No Hit
GGTTATTCCCCGGCCCACTACTGGTTCGACCCCTGGGGCCAGGGAACCCT14190.1392841052198657No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT13970.13712466172808485No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC13900.13643756607160912No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13880.13624125302690177No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCTTCTCCT13680.13427812257982827No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13650.13398365301276724No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13570.1331984008339378No Hit
CTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATTCCC13410.131627896476279No Hit
TCCCTCAAGAGTCGAGTCACCATATCAATAGACACGTCGAAGAACCAGCT13400.13152973995392533No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13210.12966476602920549No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT13020.12779979210448567No Hit
TCAATAGACACGTCGAAGAACCAGCTTTCCCTGAAGCTGAGCTCTGTGAC13020.12779979210448567No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCT12920.1268182268809489No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT12890.12652375731388787No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC12850.12613113122447317No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12740.12505140947858273No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12640.12406984425504597No Hit
GTAGTGGGCCGGGGAATAACCACTCCAAAAATCGTAATATCTCGCTCTCG12620.12387353121033863No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12420.12191040076326512No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12210.11984911379383793No Hit
TATCAATAGACACGTCGAAGAACCAGCTTTCCCTGAAGCTGAGCTCTGTG12040.11818045291382545No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12020.1179841398691181No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11910.11690441812322765No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11860.11641363551145928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.1159228528996909No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11750.11533391376556884No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11740.11523575724321516No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG11680.11464681810909312No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11650.11435234854203209No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC11230.11022977460317773No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11210.11003346155847038No Hit
GGTTTCTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGGCCGGAGTG11210.11003346155847038No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA11180.10973899199140935No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10720.10522379196314026No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10590.10394775717254248No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC10510.10316250499371309No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA10470.10276987890429838No Hit
CCATATCAATAGACACGTCGAAGAACCAGCTTTCCCTGAAGCTGAGCTCT10400.10208278324782265No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10370.10178831368076163No Hit
GCCCACTACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCTCCGTCTC10320.10129753106899322No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10310.10119937454663955No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG10260.10070859193487118No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC10210.1002178093231028No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10200.10011965280074912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)