FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005906
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT40590.4035168296043567No Hit
GTATGATAATCTCCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCA33700.33502136382524805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32990.327963050225369No Hit
GTAGTACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA32620.3242847741240235No Hit
GTGTACTACTGTGTGAATTCTGGTGGGAGCTACTCTGAGTACTGGGGCCA32530.32339005831558815No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCT28960.2878996645809847No Hit
ATATTACTGTCAACAGTATGATAATCTCCCTCGGACTTTCGGCGGAGGGA25590.2543975281984599No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAGCAA24890.2474386274661847No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22330.22198893335957834No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21830.21701828997938177No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCC21190.21065586645273018No Hit
ACATATTACTGTCAACAGTATGATAATCTCCCTCGGACTTTCGGCGGAGG20520.20399520432326676No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTGTGG20430.2031004885148314No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19270.1915685958727754No Hit
TATAAAAGGTGTCCAGTGTCAGGCGCAGCTGGTGGAGTCTGGGGGAGGCT18160.18053376756873904No Hit
TCCCAGCACGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACC18070.17963905176030365No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17860.17755138154062108No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17540.1743701697772953No Hit
ACTGTCAACAGTATGATAATCTCCCTCGGACTTTCGGCGGAGGGACCAAG17140.17039365507313806No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16760.16661596610418866No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16720.16621831463377293No Hit
GTCTCCATCCTCCCTGCCTGCATCTGTAGGAGACAGAGTCACCATCACTT16220.16124767125357636No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCCTC15850.15756939515223092No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15750.15657526647619163No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.15647585360858768No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15670.15577996353536017No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA15220.15130638449318326No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14870.14782693412704567No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14660.1457392639073631No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT14460.1437510065552845No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14280.14196157493841374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13960.13878036317508793No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13880.1379850602342565No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13840.13758740876384076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.13758740876384076No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13370.13291500398645598No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13120.1304296822963577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13040.12963437935552627No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC12610.12535962604855722No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12490.12416667163731004No Hit
CCACTGAACCGTGCTGGGACCCCTGTTGCCAAATTGGAAGCATCGTAGAT12480.12406725876970612No Hit
GTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTG12280.1220790014176275No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTGTGGCTCTCAGGTGCCAGA12170.12098545987398425No Hit
GCTGTGTACTACTGTGTGAATTCTGGTGGGAGCTACTCTGAGTACTGGGG11740.11671070656701521No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC11620.11551775215576804No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11520.11452362347972873No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11410.11343008193608548No Hit
GTACTACTGTGTGAATTCTGGTGGGAGCTACTCTGAGTACTGGGGCCAGG10930.10865826429109678No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA10900.108360025688285No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10850.10786296135026534No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10810.10746530987984962No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10800.10736589701224566No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC10730.10667000693901817No Hit
GTACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACAGTG10470.10408527238131594No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG10440.10378703377850415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10120.10060582201517836No Hit
TACTAGTAGTTACACAAACTACGCAGACTCTGTGAAGGGCCGCTTCACCA10060.10000934480955477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)