FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058106
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTATATTACTGCAGTAGAGTCTCTGCGTTACGATTTTTTGAATGGTT21280.20111406607655566No Hit
ATATAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGA21210.20045250664867226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAT11300.035.700671
TCAACGC16300.034.8897864
CCTATAT11950.033.734863
CCCTATA12850.033.6129762
ATCAACG17050.032.932833
CAACGCA17900.031.7711455
GTATCAA21150.030.6548291
TATCAAC20450.029.5695572
CTATATT14600.029.0909524
TATAGGG16350.028.6189982
ATATAGG16300.028.285191
GTACTAG2654.8803486E-927.184471
ATAGGGC15900.027.1652013
GTACATG30500.026.2174151
ATATTAC15650.025.7592076
TACATGG30600.025.6427062
TTACACG1801.59527E-423.9959264
ATTACTG16650.023.7797498
TAGGGCT18600.023.6088964
AACGCAG24050.023.347396
Produced by FastQC (version 0.11.5)