FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024078
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGGCTGGATCCGGCA47010.45904706477436286No Hit
GTAATAAAAGGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA24390.2381654522409426No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23470.22918176154550726No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20940.20447661213306018No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19800.19334464757567296No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19800.19334464757567296No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19490.19031753440655888No Hit
CTACTGGTTCGACCCCTGGGGCCGGGGAACCCTGGTCACCGTCTCCTCAG19490.19031753440655888No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19370.189145748663676No Hit
ACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATGTCAGTAGACACGTCC18910.18465390331595835No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17440.17029952796564324No Hit
CTTTTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATT17270.16863949816322585No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15610.15242979538667953No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15360.1499885750890069No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15230.14871914053421711No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGGCT15200.1484261940984964No Hit
AACCAGTAGTGGGCCGGGGAATAACCACTCCAAAAATCGTAATATCTCGC14370.14032134271022323No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13760.134364765183902No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATGTCAGTAGACAC13130.12821289003376696No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12850.1254787233003736No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12760.12459988399321147No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12720.12420928874558385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.11737387191210044No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12020.11737387191210044No Hit
TTTTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATTC11620.11346791943582421No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11390.1112219967619654No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11360.11092905032624469No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11020.10760899072140989No Hit
GTAGTAGGTGCTCCCACCATGATAGATACTCCCAATCCACTCCAGTCCCT10920.10663250260234083No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT10900.10643720497852703No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG10880.10624190735471321No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10830.10575366329517868No Hit
ATCATGGTGGGAGCACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACC10780.10526541923564417No Hit
TTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATTCCC10720.10467952636420273No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10560.10311714537369224No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT10440.10194535963080936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10420.10175006200699556No Hit
GGATTGGGAGTATCTATCATGGTGGGAGCACCTACTACAGCCCGTCCCTC10370.10126181794746103No Hit
GGTTATTCCCCGGCCCACTACTGGTTCGACCCCTGGGGCCGGGGAACCCT10310.10067592507601959No Hit
GTAGTGGGCCGGGGAATAACCACTCCAAAAATCGTAATATCTCGCTCTCG10290.10048062745220579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)