FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024078
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGGCTGGATCCGGCA45540.4446926894240478No Hit
GTAATAAAAGGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA24030.234650095012294No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21510.2100425944117538No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21190.2069178324307328No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC19570.19109872490181412No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19130.18680217717791028No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18300.17869732578963712No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18190.17762318885866116No Hit
CTACTGGTTCGACCCCTGGGGCCGGGGAACCCTGGTCACCGTCTCCTCAG18040.17615845668005758No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17170.1676630100441568No Hit
ACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATGTCAGTAGACACGTCC15630.15262509301049335No Hit
CTTTTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATT15620.15252744419858644No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14490.1414931284531061No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13790.13465771161962273No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13630.13309533062911225No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGTTTCTACTGGGGCT13510.13192354488622937No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13130.12821289003376696No Hit
AACCAGTAGTGGGCCGGGGAATAACCACTCCAAAAATCGTAATATCTCGC13020.127138753102791No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12510.12215866369553882No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12170.11883860409070404No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11900.1162020861692176No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11850.11571384210968304No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11630.11356556824773113No Hit
TTTTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATTC11240.10975726458336181No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATGTCAGTAGACAC11220.10956196695954801No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11120.10858547884047894No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11100.10839018121666513No Hit
GTAGTAGGTGCTCCCACCATGATAGATACTCCCAATCCACTCCAGTCCCT10780.10526541923564417No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG10490.10243360369034389No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10480.10233595487843698No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC10440.10194535963080936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12700.069.6420063
GTATCAA12850.069.463391
TCAACGC12850.068.2928544
CAACGCA13050.066.695025
AACGCAG14350.060.6559336
TATCAAC15150.058.85442
GGTATCA6150.052.671391
CGCAGAG17800.048.899598
GCAGAGT21400.041.3457769
ACGCAGA22050.039.47457
CAGCGCT6800.035.9674649
GTACATG26050.033.4360581
GCAGCGC7850.032.07298
GATCGTC1352.25997E-531.971087
GGCAGCG7950.031.6694687
TACATGG26200.031.562142
TGGTTAC25250.031.05316
TTACTCC25250.030.4833189
GTTACTC25550.030.4069398
ACATGGG26250.030.1323683
Produced by FastQC (version 0.11.5)