FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919815
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACTAACGACCGGCCCTCAGGGATCCCTGGGCGATTCTCTGGCT52030.565657224550589No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC51760.5627218516766959No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCCGATGATCACTA43520.4731386202660318No Hit
GTATAAGAGATGACCAGTAAAGGGGCCTGGCTTGGCTTCTGCTGGTACCA41220.4481335920810163No Hit
GTCTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCAGTCTCCGTGGC39720.4318259650038323No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTG35640.38746921935389184No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCCG31360.3409381234269935No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26590.2890798693215484No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25290.27494659252132225No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24920.27092404450895013No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24700.2685322592042965No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA23090.25102873947478566No Hit
CTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCAGTCTCCGTGGCCC20790.2260237112897702No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC20660.2246103836097476No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCCGATGATCACTAG20510.2229796209020292No Hit
GCCCTCAGGGATCCCTGGGCGATTCTCTGGCTCCAACTCTCGGAACACGG20440.22221859830509394No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20350.2212401406804629No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20350.2212401406804629No Hit
CTCACTGCACAGTCTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCA19390.21080325935106517No Hit
CCTCTCTCACTGCACAGTCTCTGTGACCTCGTACGTGCTGACTCAGCCAC18430.2003663780216674No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA18190.19775715768931795No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA18170.19753972266162217No Hit
GGTACCAGCAGAAGCCAAGCCAGGCCCCTTTACTGGTCATCTCTTATACT17480.19003821420611752No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17240.1874289938737681No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16810.18275414077830868No Hit
GTAAAGGGGCCTGGCTTGGCTTCTGCTGGTACCAGTGCACATGTTTATCT16710.18166696563982973No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16630.1807972255290466No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16530.17971005039056767No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT16410.17840544022439295No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGGCCCTGCTGATGGTCAGGGTGG16250.17666596000282664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15830.17209982442121513No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15740.1711213667965841No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15650.17014290917195307No Hit
CATGTGCACTGGTACCAGCAGAAGCCAAGCCAGGCCCCTTTACTGGTCAT15280.166120361159581No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14720.16003218038409897No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14700.1598147453564032No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14470.15731424253790163No Hit
CCCTCAGTCTCCGTGGCCCCAGGAAAGACGGCCAGGATTTCCTGTACGGG14430.15687937248251008No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14120.15350912955322538No Hit
GCACAGTCTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCAGTCTCC13550.14731223126389545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.14231122562689236No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13040.14176763805765288No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT12940.14068046291917397No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG12690.13796252507297663No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG12350.13426612960214826No Hit
CACTAGTATCAAGCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT12270.13339638949136512No Hit
GTATCAAGCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGGC12190.13252664938058198No Hit
CCCCTTTACTGGTCATCTCTTATACTAACGACCGGCCCTCAGGGATCCCT11930.12969999402053675No Hit
ATACTAGTGATCATCGGGTATTCGGCGGAGGGACCAAACTGACCGTCCTA11860.1289389714236015No Hit
GGGTGGCCGTGTTCCGAGAGTTGGAGCCAGAGAATCGCCCAGGGATCCCT11760.12785179628512255No Hit
CTATTACTGTCAGGTGTGGCTTGATACTAGTGATCATCGGGTATTCGGCG11670.12687333866049152No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11550.1255687284943168No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG11390.12382924827275049No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGT11340.12328566070351103No Hit
CTAGTATCAAGCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC11310.12295950816196735No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGGCCCTGCTGATGGTCAGGGT11250.12230720307887999No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10960.1191543951772911No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG10850.11795850252496426No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10770.11708876241418112No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG10690.11621902230339798No Hit
CCTGAGGGCCGGTCGTTAGTATAAGAGATGACCAGTAAAGGGGCCTGGCT10660.11589286976185428No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10590.11513184716491903No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10560.11480569462337537No Hit
CGGCCACCCTGACCATCAGCAGGGCCGAAGCCGGGGATGAGGCCGACTAT10320.11219647429102592No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC10000.10871751384789333No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT9560.10393394323858603No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9470.10295548561395498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.066.315331
TCAACGC21350.055.2992134
ATCAACG21350.054.962023
TATACCG553.3329905E-452.3564575
TATCAAC22900.051.297662
CAACGCA23050.050.5961045
AACGCAG24550.047.7979286
ACGCAGA26650.044.031497
CGCAGAG27650.042.439038
GCAGAGT30750.038.3947379
GAATACG3250.035.4412969
GTACATG23900.034.4376071
TACATGG25300.032.4735452
GTGTAGA5000.030.323221
CAGAGTA30550.029.9271589
ACATGGG27600.029.474223
GTGCTAG4700.029.1864831
TATTGAG13650.028.4796145
CTTAGAC1804.964646E-627.9961623
TCCGCTA1050.00797819527.4248128
Produced by FastQC (version 0.11.5)