FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919815
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACTAACGACCGGCCCTCAGGGATCCCTGGGCGATTCTCTGGCT46530.5058625919342477No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC45450.49412110043867513No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCCGATGATCACTA35520.3861646091877171No Hit
GTATAAGAGATGACCAGTAAAGGGGCCTGGCTTGGCTTCTGCTGGTACCA35440.38529486907693394No Hit
GTCTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCAGTCTCCGTGGC34240.37224876741518675No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTG29910.32517408391904895No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCCG27850.3027782760663829No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25180.27375069986899536No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22560.24526671124084737No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21470.233416502231427No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA19930.2166740050988514No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19870.21602170001576404No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17880.19438691476003328No Hit
GCCCTCAGGGATCCCTGGGCGATTCTCTGGCTCCAACTCTCGGAACACGG17860.19416947973233747No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCCGATGATCACTAG17810.19362589216309803No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17760.19308230459385856No Hit
CTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCAGTCTCCGTGGCCC17610.19145154188614014No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16710.18166696563982973No Hit
CCTCTCTCACTGCACAGTCTCTGTGACCTCGTACGTGCTGACTCAGCCAC15920.1730782820458462No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15550.16905573403347413No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15300.1663377961872768No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15000.16307627077184No Hit
CTCACTGCACAGTCTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCA14960.16264140071644842No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14900.16198909563336106No Hit
GGTACCAGCAGAAGCCAAGCCAGGCCCCTTTACTGGTCATCTCTTATACT14430.15687937248251008No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14280.15524860977479168No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA14260.15503117474709588No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14250.154922457233248No Hit
GTAAAGGGGCCTGGCTTGGCTTCTGCTGGTACCAGTGCACATGTTTATCT14000.15220451938705065No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13910.15122606176241962No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13660.14850812391622228No Hit
CATGTGCACTGGTACCAGCAGAAGCCAAGCCAGGCCCCTTTACTGGTCAT12930.14057174540532605No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12810.13926713523915135No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12730.13839739512836818No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12570.1366579149068019No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGGCCCTGCTGATGGTCAGGGTGG12520.13611432733756243No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12510.13600560982371454No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG12010.1305697341313199No Hit
CCCCTTTACTGGTCATCTCTTATACTAACGACCGGCCCTCAGGGATCCCT11980.13024358158977623No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11870.1290476889374494No Hit
CCCTCAGTCTCCGTGGCCCCAGGAAAGACGGCCAGGATTTCCTGTACGGG11650.12665590363279575No Hit
GCACAGTCTCTGTGACCTCGTACGTGCTGACTCAGCCACCCTCAGTCTCC11490.12491642341122944No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT11040.12002413528807424No Hit
GTATCAAGCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGGC10860.11806722003881215No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGT10540.11458825959567956No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10530.11447954208183167No Hit
CACTAGTATCAAGCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT10430.11339236694335275No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9810.10665188108478335No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9770.10621701102939178No Hit
ATACTAGTGATCATCGGGTATTCGGCGGAGGGACCAAACTGACCGTCCTA9700.10545598843245653No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG9650.10491240086321706No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9560.10393394323858603No Hit
GGGTGGCCGTGTTCCGAGAGTTGGAGCCAGAGAATCGCCCAGGGATCCCT9380.10197702798932394No Hit
CTATTACTGTCAGGTGTGGCTTGATACTAGTGATCATCGGGTATTCGGCG9330.10143344042008447No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9280.10088985285084502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14550.051.9487574
ATCAACG14600.050.2916873
TATCAAC15400.048.1465762
GGTATCA6950.046.6631
CAACGCA16650.044.9643175
AACGCAG17000.044.462036
ACGCAGA18100.041.3621987
CGCAGAG19500.038.3926058
GTACATG27250.037.025971
TACATGG27600.035.471432
ACATGGG29450.033.7320443
GCAGAGT22950.032.9348379
TATTGAG12950.031.685025
TACGCCT950.004894441530.3099524
TTATTGA14250.029.299624
ATGGGAG14700.028.4026975
CATGGGG16600.027.7536934
TATACTA36150.027.4801835
CATGGGA17350.027.3837784
CTAACGA33650.026.954699
Produced by FastQC (version 0.11.5)