FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346144
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26550.19723001402524543No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG25400.1886870943970333No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18540.137726721658307No Hit
GTGATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGGTCCATGTAGA18200.13520098889866167No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16330.12130945872061236No Hit
TCTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG14620.10860650866474908No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14480.1075665010578363No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13480.10013787529417359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16300.063.5859871
TCAACGC16050.060.9230274
ATCAACG16800.058.622493
CAACGCA17300.056.527395
AACGCAG18200.054.9316526
TATCAAC19650.051.217512
GGTATCA8050.045.5995751
CGCAGAG23800.041.4036878
ACGCAGA24900.039.5746087
GTACATG34800.030.4035721
GAATACG2253.0442607E-828.771039
ACATGGG34800.028.0939393
GTATTAG7800.023.0691661
GTATAGG5100.022.5806431
GTACACG10750.022.0735195
CAGAGTA27500.021.185949
ACACGGG11100.020.7358787
ACGGGGT11500.020.6400859
TATACTG6650.020.5446175
AATACGC2455.450118E-520.5446175
Produced by FastQC (version 0.11.5)