FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960182
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27700.2884869743444472No Hit
GAGTTATAGTATGTTGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCA23710.24693235240818928No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23270.24234988783376482No Hit
ATAGTATGTTGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGA21920.22829005334405353No Hit
CTATAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACT19900.20725237507055955No Hit
CATTAGTAGTTATTTAAATTGGTATCAACAGAAACCAGGGAAAGCCCCGA17910.1865271375635036No Hit
GTTATAGTATGTTGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA17480.18204881991122518No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16570.17257144999593826No Hit
ATCTATTTCTGTGCGAGAGAAGGTTGTACTGGTACGAACTGCCACTCTTC16460.17142583385233215No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAACCCAGC15650.16298993315850538No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15340.15976137857197906No Hit
TACTATAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGA15320.15955308472768703No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14770.1538250040096565No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14760.1537208570875105No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13810.14382689948363958No Hit
ACCTACTACTGTCAACAGAGTTATAGTATGTTGTACAGTTTTGGCCAGGG13730.1429937241064715No Hit
GAAATAGATAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGGAGATATA13490.14049419797496726No Hit
ACATACTATAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGC13410.13966102259779917No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13330.1388278472206311No Hit
ACCCAGCACATTAGTAGTTATTTAAATTGGTATCAACAGAAACCAGGGAA13190.13736979031058694No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA13060.13601588032268883No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12840.1337246480354766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12670.13195415035899444No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12580.13101682805968037No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12430.1294546242274902No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11860.12351824966516764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.12164360506653946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11670.12153945814439346No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11640.12122701737795542No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA11420.11893578509074322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11120.11581137742636292No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10910.11362429206129672No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10050.10466765675673988No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9900.10310545292454973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG20050.056.423823
CAACGCA20250.055.1548735
TCAACGC20650.054.784394
AACGCAG20850.053.2193156
CGCAGAG24800.044.998218
GCAGAGT27950.039.9268579
GAATACG2109.094947E-1237.7127849
ACGCAGA29950.037.035667
CAGCGCT4750.034.8617749
GTACATG24900.033.2846221
GTGCTAG3750.032.671031
GTATCAA34650.032.654391
TACATGG25850.031.498882
TATCAAC37000.030.5756112
ACATGGG25950.030.5444663
CATGGGA14550.027.2381084
TCTACAC6400.027.021443
AGCGCTG4800.027.021445
CATGGGG13800.026.6298264
GCAGCGC6850.026.2763278
Produced by FastQC (version 0.11.5)