FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960182
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25590.2665119737716391No Hit
GAGTTATAGTATGTTGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCA23790.24776552778535735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20660.21516754115365627No Hit
ATAGTATGTTGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGA20560.21412607193219615No Hit
CTATAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACT18660.19433815672445434No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17680.18413175835414536No Hit
CATTAGTAGTTATTTAAATTGGTATCAACAGAAACCAGGGAAAGCCCCGA17090.1779870899475308No Hit
GTTATAGTATGTTGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA17020.17725806149250872No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15850.16507287160142556No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAACCCAGC15210.15840746858408092No Hit
ATCTATTTCTGTGCGAGAGAAGGTTGTACTGGTACGAACTGCCACTCTTC15080.1570535585961828No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14330.14924253943523208No Hit
TACTATAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGA14200.14788862944733394No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13960.1453891033158297No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13230.13778637799917098No Hit
GAAATAGATAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGGAGATATA13110.13653661493341887No Hit
ACCTACTACTGTCAACAGAGTTATAGTATGTTGTACAGTTTTGGCCAGGG12910.13445367649049866No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12910.13445367649049866No Hit
ACATACTATAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGC12690.13216244420328646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12610.1313292688261184No Hit
ACCCAGCACATTAGTAGTTATTTAAATTGGTATCAACAGAAACCAGGGAA12540.13060024037109633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12290.12799656731744607No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA12050.12549704118594182No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA11960.12455971888662774No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11950.12445557196448173No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11510.1198731073900573No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11360.11831090355786716No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11070.11529064281563288No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10690.11133305977408449No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10580.11018744363047839No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9840.10248057139167366No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)