FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909666
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT56580.6219865313202868No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40640.44675738128060183No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG30610.336497131914351No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29670.32616366886307724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27620.303627924974661No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCCGGTCTAGTCTAAGCCTCGTA26750.2940639751293332No Hit
CTCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26590.2923050878014568No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT26420.29043627001558814No Hit
TTATTACTGCATGCAAGGTTCACACTGGCCTCACACTCTTGGCCAGGGGA25300.2781240587204534No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23530.2586663676558209No Hit
GTGTGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC23140.2543790797941222No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22690.24943220918446993No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22060.24250659033095662No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT21470.23602069330941247No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21170.23272277956964427No Hit
GGCCAAGAGTGTGAGGCCAGTGTGAACCTTGCATGCAGTAATAAACCCCA20530.2256872302581387No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20280.22293896880833186No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA20130.22129001193844774No Hit
GTAATATACAGCCGTGTCCTCAACTCTCAGACTGCTCATTTGAAGATACA19740.21700272407674903No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC18740.20600967827752165No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18150.19952378125597747No Hit
AGTCTAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCA17760.1952364933942788No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT17730.19490670202030197No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17590.19336767560841012No Hit
GGTTCACACTGGCCTCACACTCTTGGCCAGGGGACCAAGCTGGAGATCAA17570.19314781469242556No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA17160.18864066591474232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16480.1811653947712677No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA16100.17698803736756127No Hit
TTTATGAGGTTTCTAAACGGGACTCTGGGGTCCCCGACAGATTCAGCGGC15050.1654453392783725No Hit
GTTTATTACTGCATGCAAGGTTCACACTGGCCTCACACTCTTGGCCAGGG14750.16214742553860428No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14470.1590693727148206No Hit
GGCCTCACACTCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTG14320.1574204158449365No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14190.15599131989103693No Hit
GCTAATGCTCTGGGTCCCAGGGTCCAGTGGGGATGTTGTGATGACTCAGT14060.15456222393713737No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCCGGTCTA13900.152803336609261No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC13590.1493954924115005No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGGTCCAGT13510.1485160487475623No Hit
GTTCACACTGGCCTCACACTCTTGGCCAGGGGACCAAGCTGGAGATCAAA13480.14818625737358546No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAGAGTG13400.14730681370964727No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA13190.14499827409180951No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATGAGGTTTCTAAACGGGAC13030.14323938676393314No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12760.14027126439814175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA12710.13972161210818035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.13961168165018809No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGT12650.1390620293602267No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12530.13774286386431944No Hit
GTCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCA12460.13697335065837352No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12430.1366435592843967No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12430.1366435592843967No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12200.13411515875057436No Hit
GTATATTACTGTGTGAAAGACCTACATACTCATCCTGACCGCTGGGGCCA12140.13345557600262073No Hit
GTATCAACGCAGAGTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCA12020.13213641050671346No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11430.12565051348516929No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11040.12136322562347059No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG10960.1204837819595324No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10900.11982419921157876No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG10670.11729579867775644No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCA10530.11575677226586462No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10350.11377802402200367No Hit
GTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCC10140.11146948440416593No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT9950.10938080570231272No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9830.10806164020640543No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9700.10663254425250586No Hit
GTCTAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAG9660.10619282242053678No Hit
GTATACGAGGCTTAGACTAGACCGGCAGGAGATGGAGGCCGGCTGTCCAA9650.1060828919625445No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9570.10520344829860631No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTTCACACT9540.10487365692462948No Hit
TCTCCAAGGCGCCTAATTTATGAGGTTTCTAAACGGGACTCTGGGGTCCC9540.10487365692462948No Hit
GTCCCAGGGTCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCT9450.10388428280269901No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT9430.10366442188671446No Hit
ACTCTGGGGTCCCCGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTC9410.10344456097072992No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9360.10289490868076856No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTTCACAC9310.10234525639080717No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT9260.10179560410084582No Hit
CCCTGAGACTGTCCTGTTCAGCCTCTGGATTCACCTTGAGTAGTTATTCA9200.10113602135289215No Hit
CTGTATACGAGGCTTAGACTAGACCGGCAGGAGATGGAGGCCGGCTGTCC9170.10080622997891535No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9170.10080622997891535No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGGTCCAGTGGGGATGTTG9130.10036650814694624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.089.8808441
GTATCAA21000.074.848971
TATCAAC23100.066.461912
ATCAACG23750.064.339463
TCAACGC24300.062.8832174
CAACGCA24900.061.3679545
AACGCAG26350.058.194056
ACGCAGA29500.051.9743967
CGCAGAG30100.050.9383628
GCAGAGT34800.044.2656069
GAATACG3300.034.9019
CCGTTCA850.002839694733.8745049
GTACATG26900.033.5047451
CTAGTAC1301.7219854E-533.2669563
TACATGG27450.033.0851672
ACATTAG10000.032.392497
CTAGAGA5400.030.700064
CTCCAAC10900.029.7571372
GTGTAGA3600.028.0397511
ACCGTTC1050.00798183727.4222158
Produced by FastQC (version 0.11.5)