FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909666
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT51820.5696596333159643No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35890.3945404137342717No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27540.30274848131072285No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG25940.285159608031959No Hit
CTCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25560.28098225062825255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24380.26801045658516426No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCCGGTCTAGTCTAAGCCTCGTA23700.2605351854416896No Hit
TTATTACTGCATGCAAGGTTCACACTGGCCTCACACTCTTGGCCAGGGGA23550.2588862285718055No Hit
GTGTGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC22590.24833290460454716No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT22300.24514492132277121No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22090.24283638170493346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20380.22403827338825458No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA20260.2227191078923473No Hit
GGCCAAGAGTGTGAGGCCAGTGTGAACCTTGCATGCAGTAATAAACCCCA19960.21942119415257907No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19860.21832188957265636No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT19380.21304522758902716No Hit
GTAATATACAGCCGTGTCCTCAACTCTCAGACTGCTCATTTGAAGATACA19300.212165783925089No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18530.20370113865968387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18260.20073301629389248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17460.19193857965451055No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC17220.18930024866269599No Hit
GGTTCACACTGGCCTCACACTCTTGGCCAGGGGACCAAGCTGGAGATCAA17200.18908038774671143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17180.18886052683072688No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA17170.1887505963727346No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT16980.1866619176708814No Hit
AGTCTAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCA16720.18380372576308227No Hit
GTTTATTACTGCATGCAAGGTTCACACTGGCCTCACACTCTTGGCCAGGG15320.1684134616441639No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14940.16423610424045748No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG13760.15126431019736913No Hit
GTATATTACTGTGTGAAAGACCTACATACTCATCCTGACCGCTGGGGCCA13740.1510444492813846No Hit
GGCCTCACACTCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTG13590.1493954924115005No Hit
TTTATGAGGTTTCTAAACGGGACTCTGGGGTCCCCGACAGATTCAGCGGC13450.14785646599960867No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCCGGTCTA13320.14642737004570908No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13100.14400889996987906No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13030.14323938676393314No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13020.14312945630594087No Hit
GTTCACACTGGCCTCACACTCTTGGCCAGGGGACCAAGCTGGAGATCAAA13000.14290959538995632No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12910.14192022126802584No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGT12880.14159042989404902No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAGAGTG12840.14115070806207994No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATGAGGTTTCTAAACGGGAC12420.1365336288264044No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12330.13554425470447395No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12090.13290592371265939No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12030.13224634096470572No Hit
GTCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCA11870.13048745363682934No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11870.13048745363682934No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11570.1271895398970611No Hit
GCTAATGCTCTGGGTCCCAGGGTCCAGTGGGGATGTTGTGATGACTCAGT11470.12609023531713837No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG10970.12059371241752467No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGGTCCAGT10940.12026392104354784No Hit
GTATCAACGCAGAGTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCA10890.11971426875358648No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG10540.1158667027238569No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10460.11498725905991869No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10170.11179927577814273No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10060.11059004074022773No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9940.10927087524432044No Hit
GTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCC9830.10806164020640543No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9600.10553323967258312No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9400.10333463051273764No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9280.10201546501683034No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9260.10179560410084582No Hit
GTCTAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAG9240.10157574318486126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9160.10069629952092307No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG9140.10047643860493852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14600.068.001271
ATCAACG15300.065.3135153
TCAACGC15300.065.3135154
TATCAAC16800.059.0670362
CAACGCA17050.058.6097765
AACGCAG18000.055.492026
GGTATCA4650.052.6037561
ACGCAGA19500.050.5978437
CGCAGAG19800.049.8312038
GCAGAGT23800.040.8487559
GTGCTAG4400.039.241841
CAGCCGT9350.038.5106129
GTAATAT10300.037.7178841
GTACATG26850.034.832921
ACCGACC2353.6379788E-1133.7090769
TACATGG27400.033.329482
TAATATA12250.032.872092
AATATAC12100.030.895673
GAATACG4200.030.8635089
TAGCGGC1000.006275002428.8075287
Produced by FastQC (version 0.11.5)