FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585141
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCAGATCTCAGCCTGCTCAGCTCCATGTAAA21830.37307247313040787No Hit
ATATATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAGTGG21390.3655529180146324No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT20320.3472667271649056No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19280.3294932332548907No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13180.22524485551345744No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG12480.21328192692017822No Hit
GCCATATATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAG11860.2026861901661309No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11840.20234439220632294No Hit
ACCTAGGGCCACTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG11760.200977200367091No Hit
TTATTACTGCATGCAAGCTCTACAAACTTGGACGTTCGGCCAAGGGACCA11050.18884337279390778No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10830.18508359523602003No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA10720.18320370645707615No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10050.1717534748035089No Hit
ATATATGGCCGTGTCCTCAGATCTCAGCCTGCTCAGCTCCATGTAAAGTA9740.16645560642648524No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9390.16047414212984562No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9190.15705616253176582No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8700.14868211251647037No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG8660.14799851659685442No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8600.1469731227174305No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8510.1454350318982946No Hit
ATATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAGTGGCC8480.14492233495858264No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT8300.14184615332031084No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8130.13894087066194302No Hit
GCCCTAGGTTCCACTACTACTACGGTATCGACGTCTGGGGCCAAGGGACC8120.13876997168203903No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7800.1333012043251114No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7790.1331303053452074No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7510.1283451339078957No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC7500.1281742349279917No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7490.12800333594808772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.12578164920933585No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG7350.12561075022943188No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC7290.12458535635000793No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6900.11792029613375238No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG6880.11757849817394439No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG6750.11535681143519255No Hit
CAGTAATAAATGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT6660.11381872061605663No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT6620.11313512469644069No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6490.11091343795768882No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6200.10595736754047316No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6160.1052737716208572No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6100.10424837774143327No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6030.10305208488210535No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT5930.10134309508306545No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC5930.10134309508306545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4450.081.097391
GTATCAA11050.078.381911
ATCAACG13500.062.963653
TCAACGC13800.061.5948754
CAACGCA13850.061.3725135
TATCAAC13900.060.633512
AACGCAG14600.057.672166
GGCGCGA400.006228834453.9752246
CAGCGCT3450.050.0639769
CGCAGAG17600.048.250588
ACGCAGA19550.043.0697487
GCAGCGC4350.039.7059178
GTACATG17750.035.7833671
GGCAGCG4700.035.217887
GTGTAGA2053.0559022E-1035.2081381
ACATGGG18000.034.4165733
CATGGGT3050.033.0650984
GTATTAG2455.820766E-1132.4058571
GTACACG5800.032.2914125
CATGGGA8450.031.5418134
Produced by FastQC (version 0.11.5)