FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585141
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCAGATCTCAGCCTGCTCAGCTCCATGTAAA22670.38742798744234297No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT18380.31411232506353165No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17640.30146580055063654No Hit
ATATATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAGTGG17030.29104096277649316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12270.20969304834219443No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG11440.19550843301016338No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11360.19414124117093146No Hit
ACCTAGGGCCACTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG10750.18371640339678813No Hit
TTATTACTGCATGCAAGCTCTACAAACTTGGACGTTCGGCCAAGGGACCA10670.1823492115575562No Hit
ATATATGGCCGTGTCCTCAGATCTCAGCCTGCTCAGCTCCATGTAAAGTA10240.1750005554216847No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10180.17397516154226075No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA10080.17226617174322087No Hit
GCCATATATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAG9650.16491751560734935No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9470.16184133396907754No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8760.14970750639589434No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC8700.14868211251647037No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8650.14782761761695046No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8010.13689008290309515No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7960.1360355880035752No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG7860.13432659820453532No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7640.13056682064664757No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7390.12629434614904783No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7340.1254398512495279No Hit
GCCCTAGGTTCCACTACTACTACGGTATCGACGTCTGGGGCCAAGGGACC7320.1250980532897199No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC7150.12219277063135209No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT7130.12185097267154413No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG6990.11945838695288828No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6980.11928748797298427No Hit
ATATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGAGTGGCC6800.11621130633471248No Hit
CAGTAATAAATGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT6640.11347692265624867No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC6440.11005894305816889No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG6410.10954624611845692No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6400.10937534713855294No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG6340.108349953259129No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6180.10561556958066518No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT6170.10544467060076118No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6170.10544467060076118No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT6010.10271028692229736No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6000.10253938794239337No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6000.10253938794239337No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5870.10031770120364152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)