FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616274
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATAATCCGTCCCTCAGGAGTCGAATCAGCATCTCCGTTGATACGTCC27720.4497999266560004No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16480.2674135206093393No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15560.25248509591512863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.24128877739447063No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14780.2398283880222109No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12990.2107828660628227No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12940.2099715386337895No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11920.19342045908151245No Hit
TACTTAGGCTGGTATCAGCAAAAACCAGGGAAAGCCCCTATGCTCCTGAT11680.18952608742215313No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11590.1880656980498934No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11470.18611851222021372No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11400.18498265381956727No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11080.17979015827375486No Hit
GATCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG10450.16956743266793667No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10280.16680891940922382No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10100.16388814066470433No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTGAGAC9960.1616164238634114No Hit
GGTCTAGAGTGGATTGGAAGTATTTTTTATACTGGGAGCACCTACTATAA9060.14701253014081397No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8700.14117097265177503No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8370.13581621162015597No Hit
GTGTATTTCTGTGCGAGACAAAATGAGAACTGGTTCGACCGCTGGGGCCA8350.1354916806485427No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8120.13175957447499No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8090.1312727780175701No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7870.12770293732982407No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7830.12705387538659751No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTGCTCTGG7600.12332176921304484No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7590.1231595037272382No Hit
CCCTTAAACTGAGGTCTGTGACCGCCGCAGACACGGCTGTGTATTTCTGT7480.12137458338336518No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7460.12105005241175192No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7440.12072552144013864No Hit
GTACTAGGAGTTTTTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGT7430.120563255954332No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7360.11942739755368553No Hit
TCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGC7360.11942739755368553No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7350.11926513206787889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.11910286658207225No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7330.11894060109626561No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7320.11877833561045897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.11796700818142579No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7240.11748021172400588No Hit
GTCTAGAGTGGATTGGAAGTATTTTTTATACTGGGAGCACCTACTATAAT6890.11180091972077355No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC6590.10693295514657442No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA6460.10482350383108811No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6360.10320084897302173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6300.1022272560581819No Hit
GAAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCAGTTTAAGGGAGA6290.10206499057237527No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6290.10206499057237527No Hit
GTCTCCGGTGGCTCCATCAGTACTAGGAGTTTTTACTGGGGCTGGGTCCG6230.10109139765753544No Hit
CCGTTGATACGTCCAAGAATCACTTCTCCCTTAAACTGAGGTCTGTGACC6170.10011780474269562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)