FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553910
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCC36410.2343121545005824No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35310.22723323744618415No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT34350.22105527347143658No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGG31730.20419458012368796No Hit
ATAATAATCACCCTCATCTGCAGACTGGAGCCCACTGATGGCCAGGGAGG30820.19833838510595853No Hit
ATACCACACCACTCAGGCTGTCATCCCATGCTGCACAATAATAATCACCC26470.17034448584538356No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25490.16403781428782876No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC23640.15213236287815898No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22480.14466732307533897No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22400.14415249274411002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.13971208113726022No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAG21550.13868242047480228No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21100.13578649986163935No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20770.13366282474531987No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC20410.13134608825478952No Hit
GATTATTATTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGGTATTCGG20250.13031642759233159No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20160.12973724346969903No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19390.12478200153162022No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19220.12368798707775869No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC18850.12130689679582472No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA18090.11641600864914957No Hit
GAAGTAATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAG17900.11519328661248078No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17700.11390621078440837No Hit
CAATAATAATCACCCTCATCTGCAGACTGGAGCCCACTGATGGCCAGGGA17590.11319831907896853No Hit
ATCCCATGCTGCACAATAATAATCACCCTCATCTGCAGACTGGAGCCCAC17520.11274784253914319No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17210.11075287500563095No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA17100.11004498330019112No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17000.10940144538615491No Hit
TTATTATTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGGTATTCGGCG16940.1090153226377332No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16760.10785695439246804No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16010.10303042003719651No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15980.10283735866298561No Hit
GCATGGGATGACAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCT15970.10277300487158202No Hit
GTCTATTACTGTGCGAGAGTGGAGACGAGGAGGTATAATTATGGTAATAG15900.10232252833175667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33750.065.097981
ATCAACG38550.056.5967983
TCAACGC38500.056.483274
TATCAAC41500.052.9189762
CAACGCA42600.051.045455
AACGCAG44050.049.3620036
CGCAGAG46850.046.411878
ACGCAGA49700.043.8952947
GGTATCA18300.042.905881
GCAGAGT55200.039.3912359
GTACATG49800.034.8601381
TACATGG49450.033.6360782
ACATGGG47900.032.6210443
GTATAGA5300.028.5420281
GTGTAGA8150.027.399681
TATTGAG21300.025.6917485
CATGGGG27250.025.1032964
CAGAGTA52950.024.4758769
GTCTAGT3352.2755557E-923.6531661
TTATTGA24900.023.4239064
Produced by FastQC (version 0.11.5)