FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553910
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT33080.21288234196317674No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCC33060.21275363438036954No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30650.19724437065209696No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGG28830.18553198061663803No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22650.14576133752920054No Hit
ATAATAATCACCCTCATCTGCAGACTGGAGCCCACTGATGGCCAGGGAGG21680.13951901976304934No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC21170.13623697640146468No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21110.13585085365304297No Hit
ATACCACACCACTCAGGCTGTCATCCCATGCTGCACAATAATAATCACCC20640.1328262254570728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.13031642759233159No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20220.13012336621812073No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAG19920.1281927524760121No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19170.12336621812074058No Hit
GATTATTATTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGGTATTCGG18830.1211781892130175No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18640.11995546717634868No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC18510.11911886788810162No Hit
GAAGTAATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAG18130.11667342381476405No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC17360.11171818187668525No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17310.11139641291966716No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17280.11120335154545631No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16640.10708470889562458No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16540.10644117098158837No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16510.10624810960737752No Hit
TTATTATTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGGTATTCGGCG16440.10579763306755216No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15990.10290171245438924No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)