FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755401
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTATTGTGCGAGACAACGGGACAATTGGTTCGACTCCTGGGGCCA60010.3418592105165714No Hit
ATAATATACAGCCGTGTCAGCGGCAGTCACAGTGTTCACCTTCAGTGAGA59540.3391817596093428No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT42610.2427365599085337No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41910.23874886706798049No Hit
GCTGTATATTATTGTGCGAGACAACGGGACAATTGGTTCGACTCCTGGGG40710.23191282219846063No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA40020.22798209639848674No Hit
ATATACAGCCGTGTCAGCGGCAGTCACAGTGTTCACCTTCAGTGAGAACT39390.22439317284198879No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC38650.22017761183911827No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35890.2044547086392226No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC35160.2002961146769314No Hit
ATATTATTGTGCGAGACAACGGGACAATTGGTTCGACTCCTGGGGCCAGG33330.1898711462509136No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG31320.17842077109446786No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30180.17192652846842402No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29680.16907817643945744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29200.16634375849164948No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG28200.1606470544337163No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATAAAGATAGACACGTCC27290.15546305374099706No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27210.15500731741636242No Hit
CAATAATATACAGCCGTGTCAGCGGCAGTCACAGTGTTCACCTTCAGTGA26370.1502220860076985No Hit
TCCCAGAACCAGTTCTCACTGAAGGTGAACACTGTGACTGCCGCTGACAC26220.14936758039900855No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG23690.13495491913243754No Hit
GTCCCAGATGCAGCTGCAGGAGTCGGGCCCAGGTTTGGTGAAGCCTTCGG22850.13016968772377366No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG22560.128517643546973No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22310.1270934675324897No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT22040.12555535743684776No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTCGCTACTACTGGGCCTGGATCCG21440.12213733500208784No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20460.1165545650253133No Hit
CATTTGTAGTGTCTCTGGTGGCTCCATCAGCACTAGTCGCTACTACTGGG20270.11547219125430601No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA20260.11541522421372667No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA20170.11490252084851268No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC20050.1142189163615607No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20000.11393408115866403No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG19620.11176933361664941No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19390.11045909168332477No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19330.11011728943984879No Hit
CCATAAAGATAGACACGTCCCAGAACCAGTTCTCACTGAAGGTGAACACT19310.11000335535869012No Hit
AATCTATGCTGCCTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCG19150.10909188270942081No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19040.10846524526304817No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18990.1081804100601515No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG18900.10766770669493751No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC18690.10647139884277154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18590.10590172843697823No Hit
ATATTTCACAGTGAGGACACCTACTACAACCCGTCCCTCAAGAGTCGAGT18520.10550295915292289No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT18510.10544599211234355No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18210.1037369808949636No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG18150.1033951786514876No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC18060.10288247528627362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.1026546071239563No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17740.10105952998773499No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)