FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716539
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCGACATTCAGGCTGTTCATTTGCAGAAACA25830.3604828208932103No Hit
GTCTATTACTGTGCGAGGGAGATGCTGGGCTTTGACCTCTGGGGCCAGGG19790.27618873501651686No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17540.24478779243000032No Hit
GAGTACACTCTCACCATCAGCAACCTGCAGTCTGAAGACATTGCAGTTTA17530.24464823268517136No Hit
GTCTGGGACAGAGTACACTCTCACCATCAGCAACCTGCAGTCTGAAGACA11560.16133106502228073No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGGGGCCCTGCT10300.14374653717383143No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10060.14039710329793634No Hit
GTATCTATGGGCTCCATCATACCATATGGCTGCCACCCACTCCAGCCCCT10020.13983886431862047No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC9900.13816414738067295No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9560.1334191160564882No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCT9340.13034880167025104No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9340.13034880167025104No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9090.12685980804952696No Hit
CATAGATACTATGCAGATTTTCTGAAGGGCCGATTCACCGTCTCCAGAGA8730.12183565723568432No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8000.11164779586317003No Hit
GTATGATGGAGCCCATAGATACTATGCAGATTTTCTGAAGGGCCGATTCA7660.10690276453898533No Hit
TATCATAACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAT7540.10522804760103777No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7490.10453024887689295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7430.10369289040791918No Hit
GTCTGAAGACATTGCAGTTTATTACTGTCAGCAATATCATAACTGGCCTC7350.10257641244928747No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7250.10118081500099785No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)