FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226477
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36110.29442052317328415No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26020.21215236812431051No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGA22290.18174005709034904No Hit
GATCTATGCTGCATCCAATTTGCAAGGTGGGGTCCCATCAAGGTTTAGTG20660.16844995870285379No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19530.15923657761213622No Hit
GTCTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA19260.15703515027187626No Hit
GTATATTACTGTGCGCGGGCTTATATTGTAGTAGTTCCAACTGCTATGGA18300.1492078530620631No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18000.1467618226839965No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA16560.1350208768692768No Hit
ATATAAGCCCGCGCACAGTAATATACGGCCGTGTCCTCAGATCTCAGGCT16250.13249331214527466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.1306180221887569No Hit
GGCTTATATTGTAGTAGTTCCAACTGCTATGGATGCTTTTGATATCTGGG15850.12923193830785248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15540.1267043735838503No Hit
GTATTTAAATTGGTATCAGCACCAACCAGGAAAAACCCCTAAGCTCCTGA15430.12580749577855924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15070.1228722593248793No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15050.1227091906330082No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14640.1193662824496505No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14520.11838787029842385No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTT14390.11732792380126167No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14010.11422961865571063No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14000.11414808430977508No Hit
ATATTACTGTGCGCGGGCTTATATTGTAGTAGTTCCAACTGCTATGGATG13450.10966369528331962No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13390.1091744892077063No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13350.10884835182396407No Hit
ACTATATGCACTGGATTCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG13310.10852221444022188No Hit
GAGTTACAGCAACTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCA13280.10827761140241521No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAGGTGGGGTCCCATCAAGGTT13170.10738073359712412No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13170.10738073359712412No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12670.10330401630034645No Hit
ACTTACTACTGTCAACAGAGTTACAGCAACTGGTACAGTTTTGGCCAGGG12510.10199946676537758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.083.17861
TCAACGC27300.066.494084
ATCAACG27750.065.41583
CAACGCA28250.064.2585
AACGCAG29800.060.8585976
CGCAGAG35050.051.5375028
ACGCAGA36650.049.287577
TATCAAC42350.043.034052
CAGCGCT6700.037.5950749
GCAGAGT54600.033.215879
GCAGCGC8000.030.5862798
GTACATG34400.029.998751
ACATGGG33650.029.541953
GGCAGCG8350.028.4423317
CATGGGA18250.025.6563934
GTATGTA11450.025.210421
ATAGTAC4900.024.9918213
GGTATCA29850.024.6593381
GTACACG8600.024.2909585
CAGAGTA42850.024.1851839
Produced by FastQC (version 0.11.5)