FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226477
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34460.28096735609391776No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25180.20530348306572402No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGA22560.18394148443060898No Hit
GTCTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA19340.1576874250393607No Hit
GTATATTACTGTGCGCGGGCTTATATTGTAGTAGTTCCAACTGCTATGGA18660.15214308951574307No Hit
GATCTATGCTGCATCCAATTTGCAAGGTGGGGTCCCATCAAGGTTTAGTG18530.15108314301858086No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17930.14619108226244765No Hit
ATATAAGCCCGCGCACAGTAATATACGGCCGTGTCCTCAGATCTCAGGCT17010.1386899224363767No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16200.13208564041559687No Hit
GGCTTATATTGTAGTAGTTCCAACTGCTATGGATGCTTTTGATATCTGGG15380.12539982404888148No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA15370.12531828970294592No Hit
GTATTTAAATTGGTATCAGCACCAACCAGGAAAAACCCCTAAGCTCCTGA15030.12254612194113709No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14210.1158603055744217No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13950.1137404125800973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13850.11292506912074177No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTT13700.11170205393170847No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13580.11072364178048183No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13500.1100713670129974No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13050.10640232144589748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13000.1059946497162197No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAGGTGGGGTCCCATCAAGGTT12850.10477163452718641No Hit
ATATTACTGTGCGCGGGCTTATATTGTAGTAGTTCCAACTGCTATGGATG12620.10289634457066868No Hit
GAGTTACAGCAACTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCA12550.1023256041491198No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12310.1003687798466665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.067.3946151
TCAACGC16300.055.584864
ATCAACG17600.052.700553
CAACGCA17300.052.3825535
AACGCAG18550.049.2404566
CGCAGAG22450.040.366078
ACGCAGA24500.036.6949427
CAGCGCT6550.032.9414149
GTACATG30050.030.9040641
TATCAAC32650.028.84872
GCAGCGC7900.028.2225888
CCGCGCA11700.027.0476199
ACATGGG33100.026.5014693
GGCAGCG8150.026.4743867
CCCGCGC12500.025.891958
TAAGCCC12000.025.7668174
GCCCGCG12350.025.624067
AGCCCGC12700.024.9178876
ATAAGCC13800.024.4882013
AAGCCCG12950.024.4368465
Produced by FastQC (version 0.11.5)